TY  - JOUR
A1  - Zühlke, Martin
A1  - Riebe, Daniel
A1  - Beitz, Toralf
A1  - Löhmannsröben, Hans-Gerd
A1  - Andreotti, Sandro
A1  - Reinert, Knut
A1  - Zenichowski, Karl
A1  - Diener, Marc
T1  - High-performance liquid chromatography with electrospray ionization ion mobility spectrometry: Characterization, data management, and applications
T2  - Journal of separation science
N2  - The combination of high-performance liquid chromatography and electrospray ionization ion mobility spectrometry facilitates the two-dimensional separation of complex mixtures in the retention and drift time plane. The ion mobility spectrometer presented here was optimized for flow rates customarily used in high-performance liquid chromatography between 100 and 1500 mu L/min. The characterization of the system with respect to such parameters as the peak capacity of each time dimension and of the 2D spectrum was carried out based on a separation of a pesticide mixture containing 24 substances. While the total ion current chromatogram is coarsely resolved, exhibiting coelutions for a number of compounds, all substances can be separately detected in the 2D plane due to the orthogonality of the separations in retention and drift dimensions. Another major advantage of the ion mobility detector is the identification of substances based on their characteristic mobilities. Electrospray ionization allows the detection of substances lacking a chromophore. As an example, the separation of a mixture of 18 amino acids is presented. A software built upon the free mass spectrometry package OpenMS was developed for processing the extensive 2D data. The different processing steps are implemented as separate modules which can be arranged in a graphic workflow facilitating automated processing of data.
KW  - Amino acids
KW  - Electrospray ionization
KW  - Ion mobility spectrometry
KW  - Pesticides
KW  - Two-dimensional separations
Y1  - 2016
UR  - https://publishup.uni-potsdam.de/frontdoor/index/index/docId/44664
SN  - 1615-9306
SN  - 1615-9314
VL  - 39
SP  - 4756
EP  - 4764
PB  - Wiley-VCH
CY  - Weinheim
ER  -