TY  - JOUR
A1  - Villatoro, José Andrés
A1  - Zühlke, Martin
A1  - Riebe, Daniel
A1  - Riedel, Jens
A1  - Beitz, Toralf
A1  - Löhmannsröben, Hans-Gerd
T1  - IR-MALDI ion mobility spectrometry
T2  - Analytical and bioanalytical chemistry : a merger of Fresenius' journal of analytical chemistry and Analusis
N2  - The novel combination of infrared matrix-assisted laser dispersion and ionization (IR-MALDI) with ion mobility (IM) spectrometry makes it possible to investigate biomolecules in their natural environment, liquid water. As an alternative to an ESI source, the IR-MALDI source was implemented in an in-house-developed ion mobility (IM) spectrometer. The release of ions directly from an aqueous solution is based on a phase explosion, induced by the absorption of an IR laser pulse (lambda = 2.94 mu m, 6 ns pulse width), which disperses the liquid as nano- and micro-droplets. The prerequisites for the application of IR-MALDI-IM spectrometry as an analytical method are narrow analyte ion signal peaks for a high spectrometer resolution. This can only be achieved by improving the desolvation of ions. One way to full desolvation is to give the cluster ions sufficient time to desolvate. Two methods for achieving this are studied: the implementation of an additional drift tube, as in ESI-IM-spectrometry, and the delayed extraction of the ions. As a result of this optimization procedure, limits of detection between 5 nM and 2.5 mu M as well as linear dynamic ranges of 2-3 orders of magnitude were obtained for a number of substances. The ability of this method to analyze simple mixtures is illustrated by the separation of two different surfactant mixtures.
KW  - Ion mobility spectrometry
KW  - IR-MALDI
KW  - Laser
Y1  - 2016
UR  - https://publishup.uni-potsdam.de/frontdoor/index/index/docId/45021
SN  - 1618-2642
SN  - 1618-2650
VL  - 408
SP  - 6259
EP  - 6268
PB  - Springer
CY  - Heidelberg
ER  -