TY  - JOUR
A1  - Teller, C.
A1  - Halamek, Jan
A1  - Makower, Alexander
A1  - Fournier, Didier
A1  - Schulze, H.
A1  - Scheller, Frieder W.
T1  - A piezoelectric sensor with propidium as a recognition element for cholinesterases
N2  - A piezoelectric biosensor has been developed on the basis of the reversible acetylcholinesterase (AChE) inhibitor propidium. The propidium cation was bound to a 11-mercaptoundecanoic acid monolayer on gold-coated quartz crystals. The immobilization was done via activation of carboxyl groups by 1,3-dicyclohexylcarbodiimide (DCC). Different types of cholinesterases (acetyl- and butyryl-ChE) from different origins were tested for their binding ability towards the immobilized propidium. Binding Studies were performed in a flow system, Furthermore, catalytically active and organophosphate-inhibited enzyme were compared re-aiding their binding capability. The binding constants were derived by using an one to one binding model and a refined model also including rebinding effects. It was shown that organophosphorylation of the active site hardly influences the affinity of AChE towards propidium. Furthermore the propidium-based biosensor provides equal sensitivity as compared with piezolelectric sensors with immobilized paraoxon- an active site ligand of AChE. (c) 2005 Elsevier B.V. All rights reserved
Y1  - 2006
UR  - https://publishup.uni-potsdam.de/frontdoor/index/index/docId/12143
ER  -