TY  - JOUR
A1  - Hofreiter, Michael
A1  - Hartmann, Stefanie
T1  - Reconstructing protein-coding sequences from ancient DNA
T2  - Odorant binding and chemosensory proteins
N2  - Obtaining information about functional details of proteins of extinct species is of critical importance for a better understanding of the real-life appearance, behavior and ecology of these lost entries in the book of life. In this chapter, we discuss the possibilities to retrieve the necessary DNA sequence information from paleogenomic data obtained from fossil specimens, which can then be used to express and subsequently analyze the protein of interest. We discuss the problems specific to ancient DNA, including mis-coding lesions, short read length and incomplete paleogenome assemblies. Finally, we discuss an alternative, but currently rarely used approach, direct PCR amplification, which is especially useful for comparatively short proteins.
KW  - re-sequencing
KW  - mapping
KW  - genome assembly
KW  - targeted assembly
KW  - SRAssembler
KW  - ancient DNA
KW  - reference sequence
KW  - paleogenomics
Y1  - 2020
UR  - https://publishup.uni-potsdam.de/frontdoor/index/index/docId/56931
SN  - 978-0-12-821157-1
SN  - 0076-6879
VL  - 642
SP  - 21
EP  - 33
PB  - Academic Press,  an imprint of Elsevier
CY  - Cambridge, MA.
ER  -