TY  - JOUR
A1  - Badalyan, Artavazd
A1  - Neumann-Schaal, Meina
A1  - Leimkühler, Silke
A1  - Wollenberger, Ursula
T1  - A Biosensor for aromatic aldehydes comprising the mediator dependent PaoABC-Aldehyde oxidoreductase
T2  - Electroanalysis : an international journal devoted to fundamental and practical aspects of electroanalysis
N2  - A novel aldehyde oxidoreductase (PaoABC) from Escherichia coli was utilized for the development of an oxygen insensitive biosensor for benzaldehyde. The enzyme was immobilized in polyvinyl alcohol and currents were measured for aldehyde oxidation with different one and two electron mediators with the highest sensitivity for benzaldehyde in the presence of hexacyanoferrate(III). The benzaldehyde biosensor was optimized with respect to mediator concentration, enzyme loading and pH using potassium hexacyanoferrate(III). The linear measuring range is between 0.5200 mu M benzaldehyde. In correspondence with the substrate selectivity of the enzyme in solution the biosensor revealed a preference for aromatic aldehydes and less effective conversion of aliphatic aldehydes. The biosensor is oxygen independent, which is a particularly attractive feature for application. The biosensor can be applied to detect contaminations with benzaldehyde in solvents such as benzyl alcohol, where traces of benzaldehyde in benzyl alcohol down to 0.0042?% can be detected.
KW  - Aldehyde oxidoreductase
KW  - Benzaldehyde
KW  - Biosensor
KW  - Aromatic aldehydes
KW  - Molybdenum cofactor
Y1  - 2013
UR  - https://publishup.uni-potsdam.de/frontdoor/index/index/docId/35402
SN  - 1040-0397
VL  - 25
IS  - 1
SP  - 101
EP  - 108
PB  - Wiley-VCH
CY  - Weinheim
ER  -