TY  - JOUR
A1  - Zhou, Ying
A1  - Zeng, Lanting
A1  - Fu, Xiumin
A1  - Mei, Xin
A1  - Cheng, Sihua
A1  - Liao, Yinyin
A1  - Deng, Rufang
A1  - Xu, Xinlan
A1  - Jiang, Yueming
A1  - Duan, Xuewu
A1  - Baldermann, Susanne
A1  - Yang, Ziyin
T1  - The sphingolipid biosynthetic enzyme Sphingolipid delta8 desaturase is important for chilling resistance of tomato
T2  - Scientific reports
N2  - The physiological functions of sphingolipids in animals have been intensively studied, while less attention has been paid to their roles in plants. Here, we reveal the involvement of sphingolipid delta8 desaturase (SlSLD) in the chilling resistance of tomato (Solanum lycopersicum cv. Micro-Tom). We used the virus-induced gene silencing (VIGS) approach to knock-down SlSLD expression in tomato leaves, and then evaluated chilling resistance. Changes in leaf cell structure under a chilling treatment were observed by transmission electron microscopy. In control plants, SlSLD was highly expressed in the fruit and leaves in response to a chilling treatment. The degree of chilling damage was greater in SlSLD-silenced plants than in control plants, indicating that SlSLD knock-down significantly reduced the chilling resistance of tomato. Compared with control plants, SlSLD-silenced plants showed higher relative electrolytic leakage and malondialdehyde content, and lower superoxide dismutase and peroxidase activities after a chilling treatment. Chilling severely damaged the chloroplasts in SlSLD-silenced plants, resulting in the disruption of chloroplast membranes, swelling of thylakoids, and reduced granal stacking. Together, these results show that SlSLD is crucial for chilling resistance in tomato.
Y1  - 2016
UR  - https://publishup.uni-potsdam.de/frontdoor/index/index/docId/44656
SN  - 2045-2322
VL  - 6
PB  - Nature Publ. Group
CY  - London
ER  -