TY  - JOUR
A1  - Moldenhawer, Ted
A1  - Moreno, Eduardo
A1  - Schindler, Daniel
A1  - Flemming, Sven
A1  - Holschneider, Matthias
A1  - Huisinga, Wilhelm
A1  - Alonso, Sergio
A1  - Beta, Carsten
T1  - Spontaneous transitions between amoeboid and keratocyte-like modes of migration
T2  - Frontiers in Cell and Developmental Biology
N2  - The motility of adherent eukaryotic cells is driven by the dynamics of the actin cytoskeleton. Despite the common force-generating actin machinery, different cell types often show diverse modes of locomotion that differ in their shape dynamics, speed, and persistence of motion. Recently, experiments in Dictyostelium discoideum have revealed that different motility modes can be induced in this model organism, depending on genetic modifications, developmental conditions, and synthetic changes of intracellular signaling. Here, we report experimental evidence that in a mutated D. discoideum cell line with increased Ras activity, switches between two distinct migratory modes, the amoeboid and fan-shaped type of locomotion, can even spontaneously occur within the same cell. We observed and characterized repeated and reversible switchings between the two modes of locomotion, suggesting that they are distinct behavioral traits that coexist within the same cell. We adapted an established phenomenological motility model that combines a reaction-diffusion system for the intracellular dynamics with a dynamic phase field to account for our experimental findings.
KW  - cell migration
KW  - amoeboid motility
KW  - keratocytle-like motility
KW  - modes of
KW  - migration
KW  - D. discoideum
KW  - actin dynamics
Y1  - 2022
UR  - https://publishup.uni-potsdam.de/frontdoor/index/index/docId/60885
SN  - 2296-634X
VL  - 10
PB  - Frontiers Media
CY  - Lausanne
ER  -