TY  - JOUR
A1  - Tada, Yuya
A1  - Grossart, Hans-Peter
T1  - Community shifts of actively growing lake bacteria after N-acetyl-glucosamine addition: improving the BrdU-FACS method
T2  - The ISME journal : multidisciplinary journal of microbial ecology
N2  - In aquatic environments, community dynamics of bacteria, especially actively growing bacteria (AGB), are tightly linked with dissolved organic matter (DOM) quantity and quality. We analyzed the community dynamics of DNA-synthesizing and accordingly AGB by linking an improved bromodeoxyuridine immunocytochemistry approach with fluorescence-activated cell sorting (BrdU-FACS). FACS-sorted cells of even oligotrophic ecosystems in winter were characterized by 16S rRNA gene analysis. In incubation experiments, we examined community shifts of AGB in response to the addition of N-acetyl-glucosamine (NAG), one of the most abundant aminosugars in aquatic systems. Our improved BrdU-FACS analysis revealed that AGB winter communities of oligotrophic Lake Stechlin (northeastern Germany) substantially differ from those of total bacteria and consist of Alpha-, Beta-, Gamma-, Deltaproteobacteria, Actinobacteria, Candidatus OP10 and Chloroflexi. AGB populations with different BrdU-fluorescence intensities and cell sizes represented different phylotypes suggesting that single-cell growth potential varies at the taxon level. NAG incubation experiments demonstrated that a variety of widespread taxa related to Alpha-, Beta-, Gammaproteobacteria, Bacteroidetes, Actinobacteria, Firmicutes, Planctomycetes, Spirochaetes, Verrucomicrobia and Chloroflexi actively grow in the presence of NAG. The BrdU-FACS approach enables detailed phylogenetic studies of AGB and, thus, to identify those phylotypes which are potential key players in aquatic DOM cycling.
KW  - actively growing bacteria (AGB)
KW  - bromodeoxyuridine (BrdU) immunocytochemistry
KW  - fluorescence-activated cell sorting (FACS)
KW  - bacterial community composition
KW  - N-acetyl-glucosamine (NAG)
KW  - Lake Stechlin
Y1  - 2014
UR  - https://publishup.uni-potsdam.de/frontdoor/index/index/docId/38117
SN  - 1751-7362
SN  - 1751-7370
VL  - 8
IS  - 2
SP  - 441
EP  - 454
PB  - Nature Publ. Group
CY  - London
ER  -