TY  - JOUR
A1  - Vogel, Stefanie
A1  - Rackwitz, Jenny
A1  - Schuerman, Robin
A1  - Prinz, Julia
A1  - Milosavljevic, Aleksandar R.
A1  - Refregiers, Matthieu
A1  - Giuliani, Alexandre
A1  - Bald, Ilko
T1  - Using DNA origami nanostructures to determine absolute cross sections for UV photon-induced DNA strand breakage
T2  - The journal of physical chemistry letters
N2  - We have characterized ultraviolet (UV) photon-induced DNA strand break processes by determination of absolute cross sections for photoabsorption and for sequence-specific DNA single strand breakage induced by photons in an energy range from 6.50 to 8.94 eV. These represent the lowest-energy photons able to induce DNA strand breaks. Oligonudeotide targets are immobilized on a UV transparent substrate in controlled quantities through attachment to DNA origami templates. Photon-induced dissociation of single DNA strands is visualized and quantified using atomic force microscopy. The obtained quantum yields for strand breakage vary between 0.06 and 0.5, indicating highly efficient DNA strand breakage by UV photons, which is clearly dependent on the photon energy. Above the ionization threshold strand breakage becomes clearly the dominant form of DNA radiation damage, which is then also dependent on the nucleotide sequence.
Y1  - 2015
UR  - https://publishup.uni-potsdam.de/frontdoor/index/index/docId/38441
SN  - 1948-7185
VL  - 6
IS  - 22
SP  - 4589
EP  - 4593
PB  - American Chemical Society
CY  - Washington
ER  -