AC electrokinetic immobilization of influenza virus
- The use of alternating current (AC) electrokinetic forces, like dielectrophoresis and AC electroosmosis, as a simple and fast method to immobilize sub-micrometer objects onto nanoelectrode arrays is presented. Due to its medical relevance, the influenza virus is chosen as a model organism. One of the outstanding features is that the immobilization of viral material to the electrodes can be achieved permanently, allowing subsequent handling independently from the electrical setup. Thus, by using merely electric fields, we demonstrate that the need of prior chemical surface modification could become obsolete. The accumulation of viral material over time is observed by fluorescence microscopy. The influences of side effects like electrothermal fluid flow, causing a fluid motion above the electrodes and causing an intensity gradient within the electrode array, are discussed. Due to the improved resolution by combining fluorescence microscopy with deconvolution, it is shown that the viral material is mainly drawn to the electrode edge andThe use of alternating current (AC) electrokinetic forces, like dielectrophoresis and AC electroosmosis, as a simple and fast method to immobilize sub-micrometer objects onto nanoelectrode arrays is presented. Due to its medical relevance, the influenza virus is chosen as a model organism. One of the outstanding features is that the immobilization of viral material to the electrodes can be achieved permanently, allowing subsequent handling independently from the electrical setup. Thus, by using merely electric fields, we demonstrate that the need of prior chemical surface modification could become obsolete. The accumulation of viral material over time is observed by fluorescence microscopy. The influences of side effects like electrothermal fluid flow, causing a fluid motion above the electrodes and causing an intensity gradient within the electrode array, are discussed. Due to the improved resolution by combining fluorescence microscopy with deconvolution, it is shown that the viral material is mainly drawn to the electrode edge and to a lesser extent to the electrode surface. Finally, areas of application for this functionalization technique are presented.…
Verfasserangaben: | Sandra StankeORCiDGND, Christian WengerORCiDGND, Frank Fabian BierORCiDGND, Ralph HölzelORCiDGND |
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DOI: | https://doi.org/10.1002/elps.202100324 |
ISSN: | 0173-0835 |
ISSN: | 1522-2683 |
Pubmed ID: | https://pubmed.ncbi.nlm.nih.gov/35307846 |
Titel des übergeordneten Werks (Englisch): | Electrophoresis : microfluids & proteomics |
Verlag: | Wiley-Blackwell |
Verlagsort: | Weinheim |
Publikationstyp: | Wissenschaftlicher Artikel |
Sprache: | Englisch |
Datum der Erstveröffentlichung: | 21.03.2022 |
Erscheinungsjahr: | 2022 |
Datum der Freischaltung: | 15.03.2024 |
Freies Schlagwort / Tag: | AC electrokinetics; AC electroosmosis; dielectrophoresis; influenza virus; nanoelectrodes |
Band: | 43 |
Ausgabe: | 12 |
Seitenanzahl: | 13 |
Erste Seite: | 1309 |
Letzte Seite: | 1321 |
Fördernde Institution: | Deutsche Forschungsgemeinschaft [SPP1857, HO1298/4-1]; European Regional; Development Fund; Ministerium fur Wissenschaft, Forschung und Kultur; [85004117]; German Academic Scholarship Foundation |
Organisationseinheiten: | Mathematisch-Naturwissenschaftliche Fakultät / Institut für Biochemie und Biologie |
DDC-Klassifikation: | 5 Naturwissenschaften und Mathematik / 54 Chemie / 540 Chemie und zugeordnete Wissenschaften |
Peer Review: | Referiert |
Publikationsweg: | Open Access / Hybrid Open-Access |
Lizenz (Deutsch): | CC-BY-NC - Namensnennung, nicht kommerziell 4.0 International |