Molecular processes studied at a single-molecule level using DNA origami nanostructures and atomic force microscopy
- DNA origami nanostructures allow for the arrangement of different functionalities such as proteins, specific DNA structures, nanoparticles, and various chemical modifications with unprecedented precision. The arranged functional entities can be visualized by atomic force microscopy (AFM) which enables the study of molecular processes at a single-molecular level. Examples comprise the investigation of chemical reactions, electron-induced bond breaking, enzymatic binding and cleavage events, and conformational transitions in DNA. In this paper, we provide an overview of the advances achieved in the field of single-molecule investigations by applying atomic force microscopy to functionalized DNA origami substrates.
| Author details: | Ilko BaldORCiDGND, Adrian Keller |
|---|---|
| DOI: | https://doi.org/10.3390/molecules190913803 |
| ISSN: | 1420-3049 |
| Pubmed ID: | https://pubmed.ncbi.nlm.nih.gov/25191873 |
| Title of parent work (English): | Molecules |
| Publisher: | MDPI |
| Place of publishing: | Basel |
| Publication type: | Review |
| Language: | English |
| Year of first publication: | 2014 |
| Publication year: | 2014 |
| Release date: | 2017/03/27 |
| Tag: | DNA origami; DNA radiation damage; G quadruplexes; atomic force microscopy; enzyme reactions; protein binding; single-molecule analysis |
| Volume: | 19 |
| Issue: | 9 |
| Number of pages: | 21 |
| First page: | 13803 |
| Last Page: | 13823 |
| Funding institution: | Marie Curie FP7 Integration Grant within the 7th European Union Framework Programme; Deutsche Forschungsgemeinschaft (DFG) |
| Organizational units: | Mathematisch-Naturwissenschaftliche Fakultät / Institut für Chemie |
| Peer review: | Referiert |
| Publishing method: | Open Access |
| External remark: | Zweitveröffentlichung in der Schriftenreihe Postprints der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe ; 1146 |
