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On-line species-unspecific isotope dilution analysis in the picomolar range reveals the time- and species-depending mercury uptake in human astrocytes

  • In order to reveal the time-depending mercury species uptake by human astrocytes, a novel approach for total mercury analysis is presented, which uses an accelerated sample introduction system combined on-line with an inductively coupled plasma mass spectrometer equipped with a collision/reaction cell. Human astrocyte samples were incubated with inorganic mercury (HgCl2), methylmercury chloride (MeHgCl), and thimerosal. After 1-h incubation with Hg2+, cellular concentrations of 3 mu M were obtained, whereas for organic species, concentrations of 14-18 mu M could be found. After 24 h, a cellular accumulation factor of 0.3 was observed for the cells incubated with Hg2+, whereas the organic species both showed values of about 5. Due to the obtained steady-state signals, reliable results with relative standard deviations of well below 5 % and limits of detection in the concentration range of 1 ng L-1 were obtained using external calibration and species-unspecific isotope dilution analysis approaches. The results were further validatedIn order to reveal the time-depending mercury species uptake by human astrocytes, a novel approach for total mercury analysis is presented, which uses an accelerated sample introduction system combined on-line with an inductively coupled plasma mass spectrometer equipped with a collision/reaction cell. Human astrocyte samples were incubated with inorganic mercury (HgCl2), methylmercury chloride (MeHgCl), and thimerosal. After 1-h incubation with Hg2+, cellular concentrations of 3 mu M were obtained, whereas for organic species, concentrations of 14-18 mu M could be found. After 24 h, a cellular accumulation factor of 0.3 was observed for the cells incubated with Hg2+, whereas the organic species both showed values of about 5. Due to the obtained steady-state signals, reliable results with relative standard deviations of well below 5 % and limits of detection in the concentration range of 1 ng L-1 were obtained using external calibration and species-unspecific isotope dilution analysis approaches. The results were further validated using atomic fluorescence spectrometry.show moreshow less

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Metadaten
Author:Christoph A. Wehe, Imke Pieper, Michael Holtkamp, Georgina M. Thyssen, Michael Sperling, Tanja Schwerdtle, Uwe Karst
DOI:https://doi.org/10.1007/s00216-013-7608-4
ISSN:1618-2642 (print)
ISSN:1618-2650 (online)
Pubmed Id:http://www.ncbi.nlm.nih.gov/pubmed?term=24442014
Parent Title (English):Analytical & bioanalytical chemistry
Publisher:Springer
Place of publication:Heidelberg
Document Type:Article
Language:English
Year of first Publication:2014
Year of Completion:2014
Release Date:2017/03/27
Tag:Astrocytes; Automation; ICP-MS; Isotope dilution analysis; Mercury; Thimerosal
Volume:406
Issue:7
Pagenumber:8
First Page:1909
Last Page:1916
Organizational units:Mathematisch-Naturwissenschaftliche Fakultät / Institut für Biochemie und Biologie
Peer Review:Referiert