Quantitative sequencing of complex mixtures of heterochitooligosaccharides by vMALDI-linear ion trap mass spectrometry
- Heterochitooligosaccharides possess interesting biol. properties. Isobaric mixts. of such linear heterochitooligosaccharides can be obtained by chem. or enzymic degrdn. of chitosan. However, the sepn. of such mixts. is a challenging anal. problem which is so far unresolved. It is shown that these isobaric mixts. can be sequenced and quantified simultaneously using std. derivatization and multistage tandem mass spectrometric techniques. A linear ion trap mass spectrometer equipped with a vacuum matrix-assisted laser desorption ionization (vMALDI) source is used to perform MS2 as well as MS3 expts. [on SciFinder (R)].
Author details: | Sophie Haebel, Sven Bahrke, Martin G. Peter |
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URL: | http://pubs.acs.org/loi/ancham |
DOI: | https://doi.org/10.1021/Ac062254u |
ISSN: | 0003-2700 |
Publication type: | Article |
Language: | English |
Year of first publication: | 2007 |
Publication year: | 2007 |
Release date: | 2017/03/25 |
Source: | Analytical chemistry. - ISSN 0003-2700. - 79 (2007), 15, S. 5557 - 5566 |
Organizational units: | Mathematisch-Naturwissenschaftliche Fakultät / Institut für Chemie |
Peer review: | Referiert |