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Streptococcus pneumoniae-induced regulation of cyclooxygenase-2 in human lung tissue

  • The majority of cases of community-acquired pneumonia are caused by Streptococcus pneumoniae and most studies on pneumococcal host interaction are based on cell culture or animal experiments. Thus, little is known about infections in human lung tissue. Cyclooxygenase-2 and its metabolites play an important regulatory role in lung inflammation. Therefore, we established a pneumococcal infection model on human lung tissue demonstrating mitogen-activated protein kinase (MAPK)-dependent induction of cyclooxygenase-2 and its related metabolites. In addition to alveolar macrophages and the vascular endothelium, cyclooxygenase-2 was upregulated in alveolar type II but not type I epithelial cells, which was confirmed in lungs of patients suffering from acute pneumonia. Moreover, we demonstrated the expression profile of all four E prostanoid receptors at the mRNA level and showed functionality of the E prostanoid(4) receptor by cyclic adenosine monophosphate production. Additionally, in comparison to previous studies,The majority of cases of community-acquired pneumonia are caused by Streptococcus pneumoniae and most studies on pneumococcal host interaction are based on cell culture or animal experiments. Thus, little is known about infections in human lung tissue. Cyclooxygenase-2 and its metabolites play an important regulatory role in lung inflammation. Therefore, we established a pneumococcal infection model on human lung tissue demonstrating mitogen-activated protein kinase (MAPK)-dependent induction of cyclooxygenase-2 and its related metabolites. In addition to alveolar macrophages and the vascular endothelium, cyclooxygenase-2 was upregulated in alveolar type II but not type I epithelial cells, which was confirmed in lungs of patients suffering from acute pneumonia. Moreover, we demonstrated the expression profile of all four E prostanoid receptors at the mRNA level and showed functionality of the E prostanoid(4) receptor by cyclic adenosine monophosphate production. Additionally, in comparison to previous studies, cyclooxygenase-2/prostaglandin E-2 related pro- and anti-inflammatory mediator regulation was partly confirmed in human lung tissue after pneumococcal infection. Overall, cell type-specific and MAPK-dependent cyclooxygenase-2 expression and prostaglandin E-2 formation in human lung tissue may play an important role in the early phase of pneumococcal infections.show moreshow less

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Author:Kolja V. Szymanski, Mario Tönnies, Anne Becher, Diana Fatykhova, Philippe D. N'Guessan, Birgitt Gutbier, Frederick Klauschen, Frank Neuschäfer-Rube, Paul Schneider, Jens Rückert, Jens Neudecker, Torsten T. Bauer, Klaus Dalhoff, Daniel Droemann, Achim D. Gruber, Olivia Kershaw, Bettina Temmesfeld-Wollbrueck, Norbert Suttorp, Stefan Hippenstiel, Andreas C. Hocke
DOI:https://doi.org/10.1183/09031936.00186911
ISSN:0903-1936 (print)
Parent Title (English):The European respiratory journal : official journal of the European Society for Clinical Respiratory Physiology
Publisher:European Respiratory Society
Place of publication:Sheffield
Document Type:Article
Language:English
Year of first Publication:2012
Year of Completion:2012
Release Date:2017/03/26
Tag:Alveolar epithelial cells; cytokines; inflammation; lung infection; pneumonia; prostaglandins
Volume:40
Issue:6
Pagenumber:10
First Page:1458
Last Page:1467
Funder:Transregional Collaborative Research Center of the Deutsche Forschungsgemeinschaft [SFB-TR84]; German Federal Ministry of Education and Research (PROGRESS - Pneumonia Research Network on Genetic Resistance and Susceptibility for the Evolution of Severe Sepsis)
Organizational units:Mathematisch-Naturwissenschaftliche Fakultät / Institut für Ernährungswissenschaft
Peer Review:Referiert