The search result changed since you submitted your search request. Documents might be displayed in a different sort order.
  • search hit 2 of 4
Back to Result List

Complementary surface-enhanced resonance raman Spectroscopic Biodetection of mixed protein solutions by Chitosan- and Silica-Coated Plasmon-Tuned Silver Nanoparticles

  • Silver nanoparticles with identical plasmonic properties but different surface functionalities are synthesized and tested as chemically selective surface-enhanced resonance Raman (SERR) amplifiers in a two-component protein solution. The surface plasmon resonances of the particles are tuned to 413 nm to match the molecular resonance of protein heme cofactors. Biocompatible functionalization of the nanoparticles with a thin film of chitosan yields selective SERR enhancement of the anionic protein cytochrome b(5), whereas functionalization with SiO2 amplifies only the spectra of the cationic protein cytochrome c. As a result, subsequent addition of the two differently functionalized particles yields complementary information on the same mixed protein sample solution. Finally, the applicability of chitosan-coated Ag nanoparticles for protein separation was tested by in situ resonance Raman spectroscopy.

Export metadata

Additional Services

Share in Twitter Search Google Scholar Statistics
Author:Arumugam Sivanesan, Govindasamy Kalaivani, Anna Fischer, Konstanze Stiba, Silke Leimkühler, Inez M. Weidinger
ISSN:0003-2700 (print)
Parent Title (English):Analytical chemistry
Publisher:American Chemical Society
Place of publication:Washington
Document Type:Article
Year of first Publication:2012
Year of Completion:2012
Release Date:2017/03/26
First Page:5759
Last Page:5764
Funder:DFG (Unicat); Fonds der Chemie; ILB (TERAsens)
Organizational units:Mathematisch-Naturwissenschaftliche Fakultät / Institut für Biochemie und Biologie
Peer Review:Referiert