Biomineralization of Engineered Spider Silk Protein-Based Composite Materials for Bone Tissue Engineering
- Materials based on biodegradable polyesters, such as poly(butylene terephthalate) (PBT) or poly(butylene terephthalate-co-poly(alkylene glycol) terephthalate) (PBTAT), have potential application as pro-regenerative scaffolds for bone tissue engineering. Herein, the preparation of films composed of PBT or PBTAT and an engineered spider silk protein, (eADF4(C16)), that displays multiple carboxylic acid moieties capable of binding calcium ions and facilitating their biomineralization with calcium carbonate or calcium phosphate is reported. Human mesenchymal stem cells cultured on films mineralized with calcium phosphate show enhanced levels of alkaline phosphatase activity suggesting that such composites have potential use for bone tissue engineering.
| Author details: | John G. Hardy, Jose Guillermo Torres-Rendon, Aldo Leal-Egana, Andreas Walther, Helmut SchlaadORCiDGND, Helmut Coelfen, Thomas R. ScheibelORCiDGND |
|---|---|
| DOI: | https://doi.org/10.3390/ma9070560 |
| ISSN: | 1996-1944 |
| Title of parent work (English): | Materials |
| Publisher: | MDPI |
| Place of publishing: | Basel |
| Publication type: | Article |
| Language: | English |
| Year of first publication: | 2016 |
| Publication year: | 2016 |
| Release date: | 2020/03/22 |
| Tag: | biodegradable polymers; biomaterials; biomineralization; bone tissue engineering; recombinant protein; spider silk |
| Volume: | 9 |
| Number of pages: | 13 |
| First page: | 93 |
| Last Page: | 108 |
| Funding institution: | Alexander von Humboldt Foundation; German Research Foundation (Deutsche Forschungsgemeinschaft) [SFB 840 TP A8] |
| Organizational units: | Mathematisch-Naturwissenschaftliche Fakultät / Institut für Chemie |
| Peer review: | Referiert |
