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Identification of whole pathogenic cells by monoclonal antibodies generated against a specific peptide from an immunogenic cell wall protein

  • We selected the immunogenic cell wall beta-(1,3)-glucosyltransferase Bgl2p from Candida albicans as a target protein for the production of antibodies. We identified a unique peptide sequence in the protein and generated monoclonal anti- C. albicans Bgl2p antibodies, which bound in particular to whole C. albicans cells.

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Author:Hani E. J. Kaba, Natalia Maier, Nicole Schliebe-Ohler, Yvonne Mayer, Peter P. Mueller, Joop van den Heuvel, Johannes Schuchhardt, Katja HanackORCiDGND, Ursula Bilitewski
DOI:https://doi.org/10.1016/j.mimet.2014.11.003
ISSN:0167-7012 (print)
ISSN:1872-8359 (online)
Pubmed Id:http://www.ncbi.nlm.nih.gov/pubmed?term=25451457
Parent Title (English):Journal of microbiological methods
Publisher:Elsevier
Place of publication:Amsterdam
Document Type:Article
Language:English
Year of first Publication:2015
Year of Completion:2015
Release Date:2017/03/27
Tag:Bgl2p; Candida; Diagnostics; Flow cytometry; Peptides
Volume:108
Pagenumber:9
First Page:61
Last Page:69
Funder:Federal Ministry of Education and Research of Germany (BMBF) through the project "The Lab in a Hankie - Impulse Centre for Integrated Bioanalysis" [03IS2201 A-N]; Helmholtz International Graduate School for Infection Research
Organizational units:Mathematisch-Naturwissenschaftliche Fakultät / Institut für Biochemie und Biologie
Peer Review:Referiert