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Identification of whole pathogenic cells by monoclonal antibodies generated against a specific peptide from an immunogenic cell wall protein
- We selected the immunogenic cell wall beta-(1,3)-glucosyltransferase Bgl2p from Candida albicans as a target protein for the production of antibodies. We identified a unique peptide sequence in the protein and generated monoclonal anti- C. albicans Bgl2p antibodies, which bound in particular to whole C. albicans cells.
| Author details: | Hani E. J. Kaba, Natalia Maier, Nicole Schliebe-Ohler, Yvonne Mayer, Peter P. Mueller, Joop van den Heuvel, Johannes Schuchhardt, Katja HanackORCiDGND, Ursula Bilitewski |
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| DOI: | https://doi.org/10.1016/j.mimet.2014.11.003 |
| ISSN: | 0167-7012 |
| ISSN: | 1872-8359 |
| Pubmed ID: | https://pubmed.ncbi.nlm.nih.gov/25451457 |
| Title of parent work (English): | Journal of microbiological methods |
| Publisher: | Elsevier |
| Place of publishing: | Amsterdam |
| Publication type: | Article |
| Language: | English |
| Year of first publication: | 2015 |
| Publication year: | 2015 |
| Release date: | 2017/03/27 |
| Tag: | Bgl2p; Candida; Diagnostics; Flow cytometry; Peptides |
| Volume: | 108 |
| Number of pages: | 9 |
| First page: | 61 |
| Last Page: | 69 |
| Funding institution: | Federal Ministry of Education and Research of Germany (BMBF) through the project "The Lab in a Hankie - Impulse Centre for Integrated Bioanalysis" [03IS2201 A-N]; Helmholtz International Graduate School for Infection Research |
| Organizational units: | Mathematisch-Naturwissenschaftliche Fakultät / Institut für Biochemie und Biologie |
| Peer review: | Referiert |
