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Retracing the molecular basis and evolutionary history of the loss of benzaldehyde emission in the genus Capsella

  • The transition from pollinator-mediated outbreeding to selfing has occurred many times in angiosperms. This is generally accompanied by a reduction in traits attracting pollinators, including reduced emission of floral scent. In Capsella, emission of benzaldehyde as a main component of floral scent has been lost in selfing C. rubella by mutation of cinnamate-CoA ligase CNL1. However, the biochemical basis and evolutionary history of this loss remain unknown, as does the reason for the absence of benzaldehyde emission in the independently derived selfer Capsella orientalis. We used plant transformation, in vitro enzyme assays, population genetics and quantitative genetics to address these questions. CNL1 has been inactivated twice independently by point mutations in C. rubella, causing a loss of enzymatic activity. Both inactive haplotypes are found within and outside of Greece, the centre of origin of C. rubella, indicating that they arose before its geographical spread. By contrast, the loss of benzaldehyde emission in C. orientalisThe transition from pollinator-mediated outbreeding to selfing has occurred many times in angiosperms. This is generally accompanied by a reduction in traits attracting pollinators, including reduced emission of floral scent. In Capsella, emission of benzaldehyde as a main component of floral scent has been lost in selfing C. rubella by mutation of cinnamate-CoA ligase CNL1. However, the biochemical basis and evolutionary history of this loss remain unknown, as does the reason for the absence of benzaldehyde emission in the independently derived selfer Capsella orientalis. We used plant transformation, in vitro enzyme assays, population genetics and quantitative genetics to address these questions. CNL1 has been inactivated twice independently by point mutations in C. rubella, causing a loss of enzymatic activity. Both inactive haplotypes are found within and outside of Greece, the centre of origin of C. rubella, indicating that they arose before its geographical spread. By contrast, the loss of benzaldehyde emission in C. orientalis is not due to an inactivating mutation in CNL1. CNL1 represents a hotspot for mutations that eliminate benzaldehyde emission, potentially reflecting the limited pleiotropy and large effect of its inactivation. Nevertheless, even closely related species have followed different evolutionary routes in reducing floral scent.show moreshow less

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Metadaten
Author:Friederike JantzenORCiD, Joseph H. LynchORCiD, Christian KappelORCiDGND, Jona Höfflin, Oded Skaliter, Natalia WozniakORCiDGND, Adrien SicardORCiD, Claudia SasGND, Funmilayo AdebesinORCiD, Jasmin Ravid, Alexander VainsteinORCiD, Monika HilkerORCiD, Natalia DudarevaORCiD, Michael LenhardORCiDGND
URN:urn:nbn:de:kobv:517-opus4-437542
DOI:https://doi.org/10.25932/publishup-43754
ISSN:1866-8372
Parent Title (German):Postprints der Universität Potsdam Mathematisch-Naturwissenschaftliche Reihe
Series (Serial Number):Postprints der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe (775)
Document Type:Postprint
Language:English
Date of first Publication:2019/11/26
Year of Completion:2019
Publishing Institution:Universität Potsdam
Release Date:2019/11/26
Tag:benzaldehyde; cinnamate-CoA ligase; evolution; floral scent; selfing syndrome; shepherd’s purse
Capsella
Issue:775
Pagenumber:12
First Page:1349
Last Page:1360
Source:New Phytologist 224 (2019) 3, S. 1349–1360 DOI: 10.1111/nph.16103
Organizational units:Mathematisch-Naturwissenschaftliche Fakultät
Dewey Decimal Classification:5 Naturwissenschaften und Mathematik / 58 Pflanzen (Botanik) / 580 Pflanzen (Botanik)
Peer Review:Referiert
Publication Way:Open Access
Grantor:DEAL Wiley
Licence (German):License LogoCreative Commons - Namensnennung, Nicht kommerziell 4.0 International