Cristina Gamba, Eppie R. Jones, Matthew D. Teasdale, Russell L. McLaughlin, Gloria M. González-Fortes, Valeria Mattiangeli, Laszlo Domboroczki, Ivett Kovari, Ildiko Pap, Alexandra Anders, Alasdair Whittle, Janos Dani, Pal Raczky, Thomas F. G. Higham, Michael Hofreiter, Daniel G. Bradley, Ron Pinhasi

• The Great Hungarian Plain was a crossroads of cultural transformations that have shaped European prehistory. Here we analyse a 5,000-year transect of human genomes, sampled from petrous bones giving consistently excellent endogenous DNA yields, from 13 Hungarian Neolithic, Copper, Bronze and Iron Age burials including two to high (similar to 22x) and seven to similar to 1x coverage, to investigate the impact of these on Europe's genetic landscape. These data suggest genomic shifts with the advent of the Neolithic, Bronze and Iron Ages, with interleaved periods of genome stability. The earliest Neolithic context genome shows a European hunter-gatherer genetic signature and a restricted ancestral population size, suggesting direct contact between cultures after the arrival of the first farmers into Europe. The latest, Iron Age, sample reveals an eastern genomic influence concordant with introduced Steppe burial rites. We observe transition towards lighter pigmentation and surprisingly, no Neolithic presence of lactase persistence.