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Applying CLSM to increment core surfaces for histometric analyses a novel advance in quantitative wood anatomy

  • A novel procedure has been developed to conduct cell structure measurements on increment core samples of conifers. The procedure combines readily available hardware and software equipment. The essential part of the procedure is the application of a confocal laser scanning microscope (CLSM) which captures images directly from increment cores surfaced with the advanced WSL core-microtome. Cell wall and lumen are displayed with a strong contrast due to the monochrome black and green nature of the images. Consecutive images are merged into long images representing entire increment cores which are then analysed for cell structures in suitable software.

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Author details:Wei Liang, Ingo Heinrich, Gerhard Helle, Isabel Dorado Linan, Thilo HeinkenORCiD
Title of parent work (English):Dendrochronologia : an interdisciplinary journal of tree-ring science
Place of publishing:Jena
Publication type:Article
Year of first publication:2013
Completion year:2013
Release date:2017/03/26
Tag:CLSM; Cell structures; Confocal laser scanning microscopy; Dendrochronology; Surface preparation; Wood anatomy
Page number:6
First page:140
Last Page:145
Funding institution:Federal Ministry of Education and Research (BMBF); Helmholtz Association of German Research Centres Initiative (Networking Fund); Helmholtz - CSC Junior Scientists Exchange Program by the Helmholtz Association of German Research Centres; China Scholarship Council (CSC)
Organizational units:Mathematisch-Naturwissenschaftliche Fakultät / Institut für Biochemie und Biologie
Peer review:Referiert