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Electrochemical switchable protein-based optical device

  • The present work contributes to the development of reusable sensing systems with a visual evaluation of the detection process related to an analyte. An electrochemical switchable protein-based optical device was designed with the core part composed of cytochrome c immobilized in a mesoporous indium tin oxide film. A color-developing redox-sensitive dye was used as switchable component of the system. The cytochrome c-catalyzed oxidation of the dye by hydrogen peroxide is spectroscopically investigated. When the dye is co-immobilized with the protein, its redox state is easily controlled by application of an electrical potential at the supporting material. This enables to electrochemically reset the system to the initial state and repetitive signal generation. The implemented reset function of the color forming reaction will make calibration of small test devices possible. The principle can be extended to other color forming redox reactions and to coupled enzyme systems, such as rapid food testing and indication of criticalThe present work contributes to the development of reusable sensing systems with a visual evaluation of the detection process related to an analyte. An electrochemical switchable protein-based optical device was designed with the core part composed of cytochrome c immobilized in a mesoporous indium tin oxide film. A color-developing redox-sensitive dye was used as switchable component of the system. The cytochrome c-catalyzed oxidation of the dye by hydrogen peroxide is spectroscopically investigated. When the dye is co-immobilized with the protein, its redox state is easily controlled by application of an electrical potential at the supporting material. This enables to electrochemically reset the system to the initial state and repetitive signal generation. The implemented reset function of the color forming reaction will make calibration of small test devices possible. The principle can be extended to other color forming redox reactions and to coupled enzyme systems, such as rapid food testing and indication of critical concentrations of metabolites for health care.show moreshow less

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Metadaten
Author:Stefano Frasca, Claudia Richter, Till von Graberg, Bernd M. Smarsly, Ursula WollenbergerORCiDGND
DOI:https://doi.org/10.1002/elsc.201100079
ISSN:1618-0240 (print)
Parent Title (English):Engineering in life sciences : Industry, Environment, Plant, Food
Publisher:Wiley-Blackwell
Place of publication:Malden
Document Type:Article
Language:English
Year of first Publication:2011
Year of Completion:2011
Release Date:2017/03/26
Tag:Cytochrome c; Electrochemical switch; Indium tin oxide; Optical device; mesoporous materials
Volume:11
Issue:6
Pagenumber:5
First Page:554
Last Page:558
Funder:Deutsche Forschungsgemeinschaft [EX314]; Bundesminister fur Bildung und Forschung [03IS2201B]
Organizational units:Mathematisch-Naturwissenschaftliche Fakultät / Institut für Biochemie und Biologie
Peer Review:Referiert