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Secretory leukocyte protease inhibitor (SLPI) might contaminate murine monoclonal antibodies after purification on protein G

  • The large scale production of a monoclonal anti-progesterone antibody in serum free medium followed by affinity chromatography on protein G lead to a contamination of the antibody sample with a protein of about 14 kDa. This protein was identified by mass spectrometry as secretory leukocyte protease inhibitor (SLPI). This SLPI contamination lead to a failure of the fiber-optic based competitive fluorescence assay to detect progesterone in milk. Purification of the monoclonal antibody using protein A columns circumvented this problem.

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Author:Joerg A. Schenk, Jörg FettkeORCiDGND, Christine Lenz, Katharina Albers, Frank Mallwitz, Nenad Gajovic-Eichelmann, Eva Ehrentreich-Förster, Emely Kusch, Frank Sellrie
DOI:https://doi.org/10.1016/j.jbiotec.2011.12.025
ISSN:0168-1656 (print)
Parent Title (English):Journal of biotechnology
Publisher:Elsevier
Place of publication:Amsterdam
Document Type:Article
Language:English
Year of first Publication:2012
Year of Completion:2012
Release Date:2017/03/26
Tag:Hybridoma; Progesterone; Protein G; SLPI; Serum-free
Volume:158
Issue:1-2
Pagenumber:2
First Page:34
Last Page:35
Organizational units:Mathematisch-Naturwissenschaftliche Fakultät / Institut für Biochemie und Biologie
Peer Review:Referiert