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Detection of progesterone in whole blood samples

  • The progesterone concentration in blood samples can be utilised as a marker for the diagnosis of early pregnancy, endocrinopathy and virilism. Here, we describe a method for progesterone detection and measurement in whole blood samples by a surface sensitive biosensor used in conjunction with an integrated optical grating coupler. This device determines refractive index changes near the biosensor's surface. Hence, biological species bound to a surface layer can be measured in real-time without any label. For the measurements, we have modified the indirect competitive immonoassay principle. The concentration of the progesterone antibody was kept at 1 µg/ml. Progesterone concentration was determined in buffer solution and whole blood in a range between 0.005 and 10 ng/ml. The detection limit was determined to be 3 pM. The relative standard deviation was calculated to be 3.5%.

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Metadaten
Author:Eva Ehrentreich-Förster, Frieder W. SchellerORCiDGND, Frank Fabian BierORCiDGND
Document Type:Article
Language:English
Year of first Publication:2003
Year of Completion:2003
Release Date:2017/03/24
Source:Biosensors & Bioelectronics. - 18 (2003), 4, S. 375 - 380
Organizational units:Mathematisch-Naturwissenschaftliche Fakultät / Institut für Biochemie und Biologie
Peer Review:Referiert