Cell-substrate impedance fluctuations of single amoeboid cells encode cell-shape and adhesion dynamics

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We show systematic electrical impedance measurements of single motile cells on microelectrodes. Wild-type cells and mutant strains were studied that differ in their cell-substrate adhesion strength. We recorded the projected cell area by time-lapse microscopy and observed irregular oscillations of the cell shape. These oscillations were correlated with long-term variations in the impedance signal. Superposed to these long-term trends, we observed fluctuations in the impedance signal. Their magnitude clearly correlated with the adhesion strength, suggesting that strongly adherent cells display more dynamic cell-substrate interactions.

Metadaten
Author details:	Helmar Leonhardt, Matthias Gerhardt, Nadine Hoeppner, Kirsten Krüger, Marco Tarantola, Carsten Beta ORCiD GND
DOI:	https://doi.org/10.1103/PhysRevE.93.012414
ISSN:	2470-0045
ISSN:	2470-0053
Pubmed ID:	https://pubmed.ncbi.nlm.nih.gov/26871108
Title of parent work (English):	Physical review : E, Statistical, nonlinear and soft matter physics
Publisher:	American Physical Society
Place of publishing:	College Park
Publication type:	Article
Language:	English
Year of first publication:	2016
Publication year:	2016
Release date:	2020/03/22
Volume:	93
Number of pages:	8
Funding institution:	Ministerium fur Wissenschaft, Forschung und Kultur des Landes Brandenburg; Europaischer Fond fur regionale Entwicklung (EFRE) [80147097, 80148364, F111-02-UNIP/003]; Deutsche Forschungsgemeinschaft [BE 3978/3-1, SFB 937]
Organizational units:	Mathematisch-Naturwissenschaftliche Fakultät / Institut für Physik und Astronomie
Peer review:	Referiert