Stimulus-induced translocation of the protein kinase A catalytic subunit to the apical membrane in blowfly salivary glands
- Secretion in blowfly (Calliphora vicina) salivary glands is regulated by the neurohormone serotonin (5-HT), which activates the InsP(3)/Ca2+ pathway and the cAMP/protein kinase A (PKA) pathway in the secretory cells. The latter signaling cascade induces the activation of a vacuolar H+-ATPase on the apical membrane. Here, we have determined the distribution of PKA by using antibodies against the PKA regulatory subunit-II (PKA-RII) and the PKA catalytic subunit (PKA-C) of Drosophila. PKA is present in high concentrations within the secretory cells. PKA-RII and PKA-C co-distribute in non-stimulated glands, being enriched in the basal portion of the secretory cells. Exposure to 8-CPT-cAMP or 5-HT induces the translocation of PKA-C to the apical membrane, whereas the PKA-RII distribution remains unchanged. The recruitment of PKA-C to the apical membrane corroborates our hypothesis that vacuolar H+-ATPase, which is enriched in this membrane domain, is a target protein for PKA.
Author details: | Martin Voss, Ruth Schmidt, Bernd Walz, Otto BaumannORCiDGND |
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URL: | http://www.springerlink.com/content/100524 |
DOI: | https://doi.org/10.1007/s00441-008-0673-x |
ISSN: | 0302-766X |
Publication type: | Article |
Language: | English |
Year of first publication: | 2009 |
Publication year: | 2009 |
Release date: | 2017/03/25 |
Source: | Cell and tissue research. - ISSN 0302-766X. - 335 (2009), 3, S. 657 - 662 |
Organizational units: | Mathematisch-Naturwissenschaftliche Fakultät / Institut für Biochemie und Biologie |
Peer review: | Referiert |