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Secretory leukocyte protease inhibitor (SLPI) might contaminate murine monoclonal antibodies after purification on protein G
- The large scale production of a monoclonal anti-progesterone antibody in serum free medium followed by affinity chromatography on protein G lead to a contamination of the antibody sample with a protein of about 14 kDa. This protein was identified by mass spectrometry as secretory leukocyte protease inhibitor (SLPI). This SLPI contamination lead to a failure of the fiber-optic based competitive fluorescence assay to detect progesterone in milk. Purification of the monoclonal antibody using protein A columns circumvented this problem.
| Author details: | Jörg A. SchenkORCiD, Jörg FettkeORCiDGND, Christine Lenz, Katharina Albers, Frank Mallwitz, Nenad Gajovic-Eichelmann, Eva Ehrentreich-Förster, Emely Kusch, Frank Sellrie |
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| DOI: | https://doi.org/10.1016/j.jbiotec.2011.12.025 |
| ISSN: | 0168-1656 |
| Title of parent work (English): | Journal of biotechnology |
| Publisher: | Elsevier |
| Place of publishing: | Amsterdam |
| Publication type: | Article |
| Language: | English |
| Year of first publication: | 2012 |
| Publication year: | 2012 |
| Release date: | 2017/03/26 |
| Tag: | Hybridoma; Progesterone; Protein G; SLPI; Serum-free |
| Volume: | 158 |
| Issue: | 1-2 |
| Number of pages: | 2 |
| First page: | 34 |
| Last Page: | 35 |
| Organizational units: | Mathematisch-Naturwissenschaftliche Fakultät / Institut für Biochemie und Biologie |
| Peer review: | Referiert |
