Meriem Durgud, Saurabh Gupta, Ivan Ivanov, Mohammad Amin Omidbakhshfard, Maria Benina, Saleh Alseekh, Nikola Staykov, Mareike Hauenstein, Paul P. Dijkwel, Stefan Hortensteiner, Valentina Toneva, Yariv Brotman, Alisdair Fernie, Bernd Müller-Röber, Tsanko S. Gechev
- The desiccation-tolerant plant Haberlea rhodopensis can withstand months of darkness without any visible senescence. Here, we investigated the molecular mechanisms of this adaptation to prolonged (30 d) darkness and subsequent return to light. H. rhodopensis plants remained green and viable throughout the dark treatment. Transcriptomic analysis revealed that darkness regulated several transcription factor (TF) genes. Stress-and autophagy-related TFs such as ERF8, HSFA2b, RD26, TGA1, and WRKY33 were up-regulated, while chloroplast-and flowering-related TFs such as ATH1, COL2, COL4, RL1, and PTAC7 were repressed. PHYTOCHROME INTERACTING FACTOR4, a negative regulator of photomorphogenesis and promoter of senescence, also was down-regulated. In response to darkness, most of the photosynthesis-and photorespiratory-related genes were strongly down-regulated, while genes related to autophagy were up-regulated. This occurred concomitant with the induction of SUCROSE NON-FERMENTING1-RELATED PROTEIN KINASES (SnRK1) signaling pathway genes,The desiccation-tolerant plant Haberlea rhodopensis can withstand months of darkness without any visible senescence. Here, we investigated the molecular mechanisms of this adaptation to prolonged (30 d) darkness and subsequent return to light. H. rhodopensis plants remained green and viable throughout the dark treatment. Transcriptomic analysis revealed that darkness regulated several transcription factor (TF) genes. Stress-and autophagy-related TFs such as ERF8, HSFA2b, RD26, TGA1, and WRKY33 were up-regulated, while chloroplast-and flowering-related TFs such as ATH1, COL2, COL4, RL1, and PTAC7 were repressed. PHYTOCHROME INTERACTING FACTOR4, a negative regulator of photomorphogenesis and promoter of senescence, also was down-regulated. In response to darkness, most of the photosynthesis-and photorespiratory-related genes were strongly down-regulated, while genes related to autophagy were up-regulated. This occurred concomitant with the induction of SUCROSE NON-FERMENTING1-RELATED PROTEIN KINASES (SnRK1) signaling pathway genes, which regulate responses to stress-induced starvation and autophagy. Most of the genes associated with chlorophyll catabolism, which are induced by darkness in dark-senescing species, were either unregulated (PHEOPHORBIDE A OXYGENASE, PAO; RED CHLOROPHYLL CATABOLITE REDUCTASE, RCCR) or repressed (STAY GREEN-LIKE, PHEOPHYTINASE, and NON-YELLOW COLORING1). Metabolite profiling revealed increases in the levels of many amino acids in darkness, suggesting increased protein degradation. In darkness, levels of the chloroplastic lipids digalactosyldiacylglycerol, monogalactosyldiacylglycerol, phosphatidylglycerol, and sulfoquinovosyldiacylglycerol decreased, while those of storage triacylglycerols increased, suggesting degradation of chloroplast membrane lipids and their conversion to triacylglycerols for use as energy and carbon sources. Collectively, these data show a coordinated response to darkness, including repression of photosynthetic, photorespiratory, flowering, and chlorophyll catabolic genes, induction of autophagy and SnRK1 pathways, and metabolic reconfigurations that enable survival under prolonged darkness.…
MetadatenAuthor details: | Meriem Durgud, Saurabh GuptaORCiD, Ivan IvanovORCiD, Mohammad Amin OmidbakhshfardORCiDGND, Maria Benina, Saleh AlseekhORCiDGND, Nikola StaykovORCiD, Mareike HauensteinORCiD, Paul P. DijkwelORCiD, Stefan HortensteinerGND, Valentina Toneva, Yariv Brotman, Alisdair FernieORCiDGND, Bernd Müller-RöberORCiDGND, Tsanko S. GechevORCiDGND |
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URN: | urn:nbn:de:kobv:517-opus4-437588 |
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DOI: | https://doi.org/10.25932/publishup-43758 |
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Title of parent work (German): | Postprints der Universität Potsdam Mathematisch-Naturwissenschaftliche Reihe |
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Publication series (Volume number): | Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe (778) |
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Publication type: | Postprint |
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Language: | English |
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Date of first publication: | 2019/11/26 |
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Publication year: | 2018 |
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Publishing institution: | Universität Potsdam |
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Release date: | 2019/11/26 |
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Tag: | arabidopsis; beta-oxidation; chlorophyll; craterostigma-plantagineum; fatty-acid; leaf senescence; lipid-metabolism; photosynthetic apparatus; stress; transcription factors |
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Issue: | 778 |
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Number of pages: | 20 |
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First page: | 1319 |
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Last Page: | 1338 |
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Source: | Plant Physiology 177 (2018) 3, S. 1319–1338 DOI: 110.1104/pp.18.00055 |
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Organizational units: | Mathematisch-Naturwissenschaftliche Fakultät |
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DDC classification: | 5 Naturwissenschaften und Mathematik / 58 Pflanzen (Botanik) / 580 Pflanzen (Botanik) |
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Peer review: | Referiert |
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Publishing method: | Open Access |
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Grantor: | Horizon 2020 |
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License (German): | Keine öffentliche Lizenz: Unter Urheberrechtsschutz |
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External remark: | Bibliographieeintrag der Originalveröffentlichung/Quelle |
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