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Analysis of polycrystallinity in hen egg-white lysozyme using a pnCCD

  • A crystal of hen egg-white lysozyme was analyzed by means of energy-dispersive X-ray Laue diffraction with white synchrotron radiation at 2.7 angstrom resolution using a pnCCD detector. From Laue spots measured in a single exposure of the arbitrarily oriented crystal, the lattice constants of the tetragonal unit cell could be extracted with an accuracy of about 2.5%. Scanning across the sample surface, Laue images with split reflections were recorded at various positions. The corresponding diffraction patterns were generated by two crystalline domains with a tilt of about 1 degrees relative to each other. The obtained results demonstrate the potential of the pnCCD for fast X-ray screening of crystals of macromolecules or proteins prior to conventional X-ray structure analysis. The described experiment can be automatized to quantitatively characterize imperfect single crystals or polycrystals.

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Author details:Sebastian Send, Ali Abboud, Wolfram LeitenbergerGND, Manfred S. Weiss, Robert Hartmann, Lothar Strüder, Ullrich Pietsch
DOI:https://doi.org/10.1107/S0021889812015038
ISSN:0021-8898
Title of parent work (English):Journal of applied crystallography
Publisher:Wiley-Blackwell
Place of publishing:Hoboken
Publication type:Article
Language:English
Year of first publication:2012
Publication year:2012
Release date:2017/03/26
Volume:45
Issue:6
Number of pages:6
First page:517
Last Page:522
Funding institution:BMBF Verbundforschung [05K10PSB]
Organizational units:Mathematisch-Naturwissenschaftliche Fakultät / Institut für Physik und Astronomie
Peer review:Referiert
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