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Monitoring cell detachment on PEG-based thermoresponsive surfaces using TIRF microscopy

  • Recently, we introduced a thermoresponsive copolymer that consists of oligo(ethylene glycol) methacrylate (OEGMA) and 2-(2- methoxyethoxy) ethyl methacrylate (MEO(2)MA). The polymer exhibited an LCST at 35 degrees C in PBS buffer and was anchored onto gold substrates using disulfide polymerisation initiators. It allows the noninvasive detachment of adherent cells from their substrate. As the mechanisms that determine the interaction of cells with such polymers are not well understood, we employed Total Internal Reflection Fluorescence (TIRF) microscopy in order to monitor the detachment process of cells of two different types. We identified contact area and average cell-substrate distance as crucial parameters for the evaluation of the detachment process. The sensitivity of TIRF microscopy allowed us to correlate the specific adhesion pattern of MCF-7 breast cancer cells with the morphology of cell deposits that may serve as fingerprints for a nondestructive characterisation of live cells.

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Metadaten
Author:Katja Uhlig, Erik Wischerhoff, Jean-Francois Lutz, André LaschewskyORCiDGND, Magnus S. Jäger, Andreas Lankenau, Claus Duschl
URL:http://www.rsc.org/Publishing/Journals/sm/index.asp
DOI:https://doi.org/10.1039/C0sm00010h
ISSN:1744-683X
Document Type:Article
Language:English
Year of first Publication:2010
Year of Completion:2010
Release Date:2017/03/25
Source:Soft matter. - ISSN 1744-683X. - 6 (2010), 17, S. 4262 - 4267
Organizational units:Mathematisch-Naturwissenschaftliche Fakultät / Institut für Chemie
Peer Review:Referiert