An optical multifrequency phase-modulation method using microbeads for measuring intracellular oxygen concentrations in plants
- A technique has been developed to measure absolute intracellular oxygen concentrations in green plants. Oxygen-sensitive phosphorescent microbeads were injected into the cells and an optical multifrequency phase-modulation technique was used to discriminate the sensor signal from the strong autofluorescence of the plant tissue. The method was established using photosynthesis-competent cells of the giant algae Chara corallina L., and was validated by application to various cell types of other plant species.
| Verfasserangaben: | Elmar Schmälzlin, Joost T. van Dongen, Ingo Klimant, Bettina Marmodée, Martin SteupORCiDGND, Joachim Fishahn, Peter Geigenberger, Hans-Gerd LöhmannsröbenORCiDGND |
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| URN: | urn:nbn:de:kobv:517-opus-12232 |
| Schriftenreihe (Bandnummer): | Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe (paper 007) |
| Publikationstyp: | Postprint |
| Sprache: | Englisch |
| Erscheinungsjahr: | 2005 |
| Veröffentlichende Institution: | Universität Potsdam |
| Datum der Freischaltung: | 26.01.2007 |
| Quelle: | Biophysical Journal. - ISSN 1542-0086 (online). - 89 (2005), S. 1339 - 1345 |
| Organisationseinheiten: | Mathematisch-Naturwissenschaftliche Fakultät / Institut für Chemie |
| DDC-Klassifikation: | 5 Naturwissenschaften und Mathematik / 54 Chemie / 540 Chemie und zugeordnete Wissenschaften |
| Externe Anmerkung: | first published in: Biophysical Journal. - ISSN 1542-0086. - 89 (2005), S. 1339 - 1345 doi: 10.1529/biophysj.105.063453 |
