Successful enrichment of the ubiquitous freshwater acI Actinobacteria
- Actinobacteria of the acI lineage are often the numerically dominant bacterial phylum in surface freshwaters, where they can account for >50% of total bacteria. Despite their abundance, there are no described isolates. In an effort to obtain enrichment of these ubiquitous freshwater Actinobacteria, diluted freshwater samples from Lake Grosse Fuchskuhle, Germany, were incubated in 96-well culture plates. With this method, a successful enrichment containing high abundances of a member of the lineage acI was established. Phylogenetic classification showed that the acIActinobacteria of the enrichment belonged to the acI-B2 tribe, which seems to prefer acidic lakes. This enrichment grows to low cell densities and thus the oligotrophic nature of acI-B2 was confirmed.
| Author details: | Sarahi L. Garcia, Katherine D. McMahon, Hans-Peter GrossartORCiDGND, Falk Warnecke |
|---|---|
| DOI: | https://doi.org/10.1111/1758-2229.12104 |
| ISSN: | 1758-2229 |
| Pubmed ID: | https://pubmed.ncbi.nlm.nih.gov/24596259 |
| Title of parent work (English): | Environmental microbiology reports |
| Publisher: | Wiley-Blackwell |
| Place of publishing: | Hoboken |
| Publication type: | Article |
| Language: | English |
| Year of first publication: | 2014 |
| Publication year: | 2014 |
| Release date: | 2017/03/27 |
| Volume: | 6 |
| Issue: | 1 |
| Number of pages: | 7 |
| First page: | 21 |
| Last Page: | 27 |
| Funding institution: | JSMC; German Science foundation [DFG GR 1540/17-1]; US National Science Foundation [CBET 0738039, MCB-0702395]; National Institute of Food and Agriculture, United States Department of Agriculture [WIS01516]; National Science Foundation [0822700] |
| Organizational units: | Mathematisch-Naturwissenschaftliche Fakultät / Institut für Biochemie und Biologie |
| Peer review: | Referiert |
