• search hit 3 of 50
Back to Result List

Human sulfite oxidase electrochemistry on gold nanoparticles modified electrode

  • The present study reports a facile approach for sulfite biosensing, based on enhanced direct electron transfer of a human sulfite oxidase (hSO) immobilized on a gold nanoparticles modified electrode. The spherical core shell AuNPs were prepared via a new method by reduction of HAuCl4 with branched poly(ethyleneimine) in an ionic liquids resulting particles with a diameter less than 10 nm. These nanoparticles were covalently attached to a mercaptoundecanoic acid modified Au-electrode where then hSO was adsorbed and an enhanced interfacial electron transfer and electrocatalysis was achieved. UV/Vis and resonance Raman spectroscopy, in combination with direct protein voltammetry, are employed for the characterization of the system and reveal no perturbation of the structural integrity of the redox protein. The proposed biosensor exhibited a quick steady-state current response, within 2 s, a linear detection range between 0.5 and 5.4 mu M with a high sensitivity (1.85 nA mu M-1). The investigated system provides remarkable advantages inThe present study reports a facile approach for sulfite biosensing, based on enhanced direct electron transfer of a human sulfite oxidase (hSO) immobilized on a gold nanoparticles modified electrode. The spherical core shell AuNPs were prepared via a new method by reduction of HAuCl4 with branched poly(ethyleneimine) in an ionic liquids resulting particles with a diameter less than 10 nm. These nanoparticles were covalently attached to a mercaptoundecanoic acid modified Au-electrode where then hSO was adsorbed and an enhanced interfacial electron transfer and electrocatalysis was achieved. UV/Vis and resonance Raman spectroscopy, in combination with direct protein voltammetry, are employed for the characterization of the system and reveal no perturbation of the structural integrity of the redox protein. The proposed biosensor exhibited a quick steady-state current response, within 2 s, a linear detection range between 0.5 and 5.4 mu M with a high sensitivity (1.85 nA mu M-1). The investigated system provides remarkable advantages in the possibility to work at low applied potential and at very high ionic strength. Therefore these properties could make the proposed system useful in the development of bioelectronic devices and its application in real samples.show moreshow less

Export metadata

Additional Services

Share in Twitter Search Google Scholar Statistics
Metadaten
Author:Stefano Frasca, Oscar Rojas, Johannes Salewski, Bettina Neumann, Konstanze Stiba, Inez M. Weidinger, Brigitte Tiersch, Silke Leimkühler, Joachim Kötz, Ursula WollenbergerORCiDGND
DOI:https://doi.org/10.1016/j.bioelechem.2011.11.012
ISSN:1567-5394 (print)
Parent Title (English):Bioelectrochemistry : an international journal devoted to electrochemical aspects of biology and biological aspects of electrochemistry ; official journal of the Bioelectrochemical Society
Publisher:Elsevier
Place of publication:Lausanne
Document Type:Article
Language:English
Year of first Publication:2012
Year of Completion:2012
Release Date:2017/03/26
Tag:Direct electron transfer; Gold nanoparticle; Human sulfite oxidase; Ionic liquid; Sulfite biosensor
Volume:87
Pagenumber:9
First Page:33
Last Page:41
Funder:Cluster of Excellence "Unifying Concepts in Catalysis"; Deutsche Forschungsgemeinschaft [EXC 314, SM 199/7-1]; ILB (TERAsens); Fonds der Chemischen Industrie; National University of Costa Rica; National Science Bureau of Costa Rica (MICIT); National Science Bureau of Costa Rica (CONICIT); DAAD
Organizational units:Mathematisch-Naturwissenschaftliche Fakultät / Institut für Biochemie und Biologie
Peer Review:Referiert