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Characterization of individual proteins in pea protein isolates and air classified samples

  • Generally, pea proteins are extracted at comparatively acidic or basic pH values to provide a basis for protein isolate production. Such processing steps result in partial denaturation of the proteins rendering them in most cases insoluble at food processing pH conditions and limiting their application in food products. Here, the comparison of the solubility properties of pea proteins in protein enriched fractions deriving from air classification is reported. Protein content, solubility, and physicochemical parameters of different fractions of the pea (Pisum sativum) variety 'Salamanca' were investigated as a function of pH using SDS-PAGE and surface hydrophobicity. Whole pea flour (20% protein), air classified, protein-enriched pea flour (48% protein), pea flour made from hulls (2.8% protein), and pea protein isolate (81% protein) served as test materials. Fractionation and pH value affected the composition and surface hydrophobicity of the proteins as well as the content of trypsin inhibitors. All samples showed a high bufferingGenerally, pea proteins are extracted at comparatively acidic or basic pH values to provide a basis for protein isolate production. Such processing steps result in partial denaturation of the proteins rendering them in most cases insoluble at food processing pH conditions and limiting their application in food products. Here, the comparison of the solubility properties of pea proteins in protein enriched fractions deriving from air classification is reported. Protein content, solubility, and physicochemical parameters of different fractions of the pea (Pisum sativum) variety 'Salamanca' were investigated as a function of pH using SDS-PAGE and surface hydrophobicity. Whole pea flour (20% protein), air classified, protein-enriched pea flour (48% protein), pea flour made from hulls (2.8% protein), and pea protein isolate (81% protein) served as test materials. Fractionation and pH value affected the composition and surface hydrophobicity of the proteins as well as the content of trypsin inhibitors. All samples showed a high buffering capacity in the range of pH 4 to 10. The direct comparison documents the comparatively better protein quality of the air classified, protein enriched pea fraction. The solubility of the pea protein isolate can be improved by using selected additives, giving new possibilities for plant protein application. Relevant technofunctional properties were determined and compared with two commercially available pea-based products (whole pea flour and an isolate). Water binding capacity was highest for the commercially available pea flour followed by the pea hull flour. Fat binding capacity remained more or less unchanged. (C) 2015 Elsevier Ltd. All rights reserved.show moreshow less

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Metadaten
Author details:Annika Reinkensmeier, Sara Bassler, Oliver Schlueter, Sascha RohnORCiDGND, Harshadrai Manilal RawelORCiDGND
DOI:https://doi.org/10.1016/j.foodres.2015.05.009
ISSN:0963-9969
ISSN:1873-7145
Title of parent work (English):Food research international
Publisher:Elsevier
Place of publishing:Amsterdam
Publication type:Article
Language:English
Year of first publication:2015
Publication year:2015
Release date:2017/03/27
Tag:Extraction; Pea flour; Pea protein isolate; Physicochemical properties; Technofunctional properties
Volume:76
Number of pages:8
First page:160
Last Page:167
Funding institution:Federal Ministry of Food and Agriculture (BMEL) [FKZ 511-06.01-28-1-54.070-10]
Organizational units:Mathematisch-Naturwissenschaftliche Fakultät / Institut für Ernährungswissenschaft
Peer review:Referiert
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