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Optical oxygen micro- and nanosensors for plant applications

  • Pioneered by Clark's microelectrode more than half a century ago, there has been substantial interest in developing new, miniaturized optical methods to detect molecular oxygen inside cells. While extensively used for animal tissue measurements, applications of intracellular optical oxygen biosensors are still scarce in plant science. A critical aspect is the strong autofluorescence of the green plant tissue that interferes with optical signals of commonly used oxygen probes. A recently developed dual-frequency phase modulation technique can overcome this limitation, offering new perspectives for plant research. This review gives an overview on the latest optical sensing techniques and methods based on phosphorescence quenching in diverse tissues and discusses the potential pitfalls for applications in plants. The most promising oxygen sensitive probes are reviewed plus different oxygen sensing structures ranging from micro-optodes to soluble nanoparticles. Moreover, the applicability of using heterologously expressed oxygen bindingPioneered by Clark's microelectrode more than half a century ago, there has been substantial interest in developing new, miniaturized optical methods to detect molecular oxygen inside cells. While extensively used for animal tissue measurements, applications of intracellular optical oxygen biosensors are still scarce in plant science. A critical aspect is the strong autofluorescence of the green plant tissue that interferes with optical signals of commonly used oxygen probes. A recently developed dual-frequency phase modulation technique can overcome this limitation, offering new perspectives for plant research. This review gives an overview on the latest optical sensing techniques and methods based on phosphorescence quenching in diverse tissues and discusses the potential pitfalls for applications in plants. The most promising oxygen sensitive probes are reviewed plus different oxygen sensing structures ranging from micro-optodes to soluble nanoparticles. Moreover, the applicability of using heterologously expressed oxygen binding proteins and fluorescent proteins to determine changes in the cellular oxygen concentration are discussed as potential non-invasive cellular oxygen reporters.show moreshow less

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Metadaten
Author:Cindy Ast, Elmar Schmälzlin, Hans-Gerd LöhmannsröbenGND, Joost T. van Dongen
DOI:https://doi.org/10.3390/s120607015
ISSN:1424-8220 (print)
Parent Title (English):Sensors
Publisher:MDPI
Place of publication:Basel
Document Type:Review
Language:English
Year of first Publication:2012
Year of Completion:2012
Release Date:2017/03/26
Tag:biosensors; dual-frequency phase-modulation; endogenous sensor proteins; microsensors; nanosensors; oxygen sensor; phosphorescence quenching; plant science
Volume:12
Issue:6
Pagenumber:18
First Page:7015
Last Page:7032
Funder:German Federal Ministry of Education and Research (BMBF) [03SF0361]
Organizational units:Mathematisch-Naturwissenschaftliche Fakultät / Institut für Chemie
Peer Review:Referiert
Publication Way:Open Access