Monitoring hormone-induced oxygen consumption in the salivary glands of the blowfly, Calliphora vicina, by use of luminescent microbeads
- The salivary glands of the blowfly were injected with luminescent oxygen-sensitive microbeads. The changes in oxygen content within individual gland tubules during hormone-induced secretory activity were quantified. The measurements are based on an upgraded phase-modulation technique, where the phase shift of the sensor phosphorescence is determined independently from concentration and background signals. We show that the combination of a lock-in amplifier with a fluorescence microscope results in a convenient setup to measure oxygen concentrations within living animal tissues at the cellular level.
Author details: | Elmar Schmälzlin, Bernd Walz, Ingo Klimant, Bettina Schewe, Hans-Gerd LöhmannsröbenORCiDGND |
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URN: | urn:nbn:de:kobv:517-opus-12206 |
Publication series (Volume number): | Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe (paper 006) |
Publication type: | Postprint |
Language: | English |
Publication year: | 2006 |
Publishing institution: | Universität Potsdam |
Release date: | 2007/01/26 |
Source: | Sensors and Actuators B: Chemical. - ISSN 0925-4005. - 119 (2006), 1 , p. 251 - 254 |
Organizational units: | Mathematisch-Naturwissenschaftliche Fakultät / Institut für Chemie |
DDC classification: | 5 Naturwissenschaften und Mathematik / 54 Chemie / 540 Chemie und zugeordnete Wissenschaften |
External remark: | first published in: Sensors and Actuators B: Chemical. - ISSN 0925-4005. - 119 (2006), 1, p. 251 - 254 doi: 10.1016/j.snb.2005.12.024 |