Utility of an appropriate reporter assay: Heliotrine interferes with GAL4/upstream activation sequence-driven reporter gene systems
- Reporter gene assays are widely used for the assessment of transcription factor activation following xenobiotic exposure of cells. A critical issue with such assays is the possibility of interference of test compounds with the test system, for example, by direct inhibition of the reporter enzyme. Here we show that the pyrrolizidine alkaloid heliotrine interferes with reporter signals derived from GAL4-based nuclear receptor transactivation assays by a mechanism independent of luciferase enzyme inhibition. These data highlight the necessity to conduct proper control experiments in order to avoid perturbation of reporter assays by test chemicals. (C) 2015 Elsevier Inc. All rights reserved.
| Author details: | Claudia Luckert, Stefanie Hessel, Alfonso Lampen, Albert Braeuning |
|---|---|
| DOI: | https://doi.org/10.1016/j.ab.2015.07.009 |
| ISSN: | 0003-2697 |
| ISSN: | 1096-0309 |
| Pubmed ID: | https://pubmed.ncbi.nlm.nih.gov/26212314 |
| Title of parent work (English): | Analytical biochemistry : methods in the biological sciences |
| Publisher: | Elsevier |
| Place of publishing: | San Diego |
| Publication type: | Article |
| Language: | English |
| Year of first publication: | 2015 |
| Publication year: | 2015 |
| Release date: | 2017/03/27 |
| Tag: | Firefly luciferase inhibition; Nuclear receptor; Transactivation assay |
| Volume: | 487 |
| Number of pages: | 4 |
| First page: | 45 |
| Last Page: | 48 |
| Funding institution: | Federal Institute for Risk Assessment [51-006, 1322-454, 1322-591] |
| Organizational units: | Mathematisch-Naturwissenschaftliche Fakultät / Institut für Ernährungswissenschaft |
| Peer review: | Referiert |
