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Polymeric sheet actuators with programmable bioinstructivity
- Stem cells are capable of sensing and processing environmental inputs, converting this information to output a specific cell lineage through signaling cascades. Despite the combinatorial nature of mechanical, thermal, and biochemical signals, these stimuli have typically been decoupled and applied independently, requiring continuous regulation by controlling units. We employ a programmable polymer actuator sheet to autonomously synchronize thermal and mechanical signals applied to mesenchymal stem cells (MSC5). Using a grid on its underside, the shape change of polymer sheet, as well as cell morphology, calcium (Ca2+) influx, and focal adhesion assembly, could be visualized and quantified. This paper gives compelling evidence that the temperature sensing and mechanosensing of MSC5 are interconnected via intracellular Ca2+. Up-regulated Ca2+ levels lead to a remarkable alteration of histone H3K9 acetylation and activation of osteogenic related genes. The interplay of physical, thermal, and biochemical signaling was utilized toStem cells are capable of sensing and processing environmental inputs, converting this information to output a specific cell lineage through signaling cascades. Despite the combinatorial nature of mechanical, thermal, and biochemical signals, these stimuli have typically been decoupled and applied independently, requiring continuous regulation by controlling units. We employ a programmable polymer actuator sheet to autonomously synchronize thermal and mechanical signals applied to mesenchymal stem cells (MSC5). Using a grid on its underside, the shape change of polymer sheet, as well as cell morphology, calcium (Ca2+) influx, and focal adhesion assembly, could be visualized and quantified. This paper gives compelling evidence that the temperature sensing and mechanosensing of MSC5 are interconnected via intracellular Ca2+. Up-regulated Ca2+ levels lead to a remarkable alteration of histone H3K9 acetylation and activation of osteogenic related genes. The interplay of physical, thermal, and biochemical signaling was utilized to accelerate the cell differentiation toward osteogenic lineage. The approach of programmable bioinstructivity provides a fundamental principle for functional biomaterials exhibiting multifaceted stimuli on differentiation programs. Technological impact is expected in the tissue engineering of periosteum for treating bone defects.…
Author details: | Zijun DengGND, Weiwei WangGND, Xun Xua, Oliver E. C. GouldORCiD, Karl KratzORCiD, Nan Ma, Andreas LendleinORCiDGND |
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DOI: | https://doi.org/10.1073/pnas.1910668117 |
ISSN: | 1091-6490 |
Pubmed ID: | https://pubmed.ncbi.nlm.nih.gov/31932451 |
Title of parent work (English): | PNAS |
Publisher: | National Academy of Sciences |
Place of publishing: | Washington, DC |
Publication type: | Article |
Language: | English |
Date of first publication: | 2020/01/13 |
Publication year: | 2020 |
Release date: | 2024/03/11 |
Tag: | HDAC1; RUNX2; calcium influx; mesenchymal stem cells; reversible shape-memory actuator |
Volume: | 117 |
Issue: | 4 |
Number of pages: | 7 |
First page: | 1895 |
Last Page: | 1901 |
Funding institution: | Helmholtz Association of German Research Centers (through program-oriented funding); Helmholtz Cross Program Initiative “Technology and Medicine Adaptive Systems”; Helmholtz Virtual Institute; Multifunctional Biomaterials for Medicine (Grant VH-VI-423); Federal Ministry of Education and Research, Germany (Grant 13GW0098, and Project 0315696A “Poly4BioBB”) |
Organizational units: | Mathematisch-Naturwissenschaftliche Fakultät / Institut für Chemie |
DDC classification: | 0 Informatik, Informationswissenschaft, allgemeine Werke / 00 Informatik, Wissen, Systeme / 000 Informatik, Informationswissenschaft, allgemeine Werke |
5 Naturwissenschaften und Mathematik / 50 Naturwissenschaften / 500 Naturwissenschaften und Mathematik | |
5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie | |
Peer review: | Referiert |
Publishing method: | Open Access / Hybrid Open-Access |
License (German): | CC-BY - Namensnennung 4.0 International |
External remark: | Zweitveröffentlichung in der Schriftenreihe Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe ; 1441 |