Recruitment of SH-Containing peptides to lipid and biological membranes through the use of a palmitic acid functionalized with a Maleimide Group
- This study presents a novel and easily applicable approach to recruit sulfhydryl-containing biomolecules to membranes by using a palmitic acid which is functionalized with a maleimide group. Notably, this strategy can also be employed with preformed (biological) membranes. The applicability of the assay is demonstrated by characterizing the binding of a Rhodamine-labeled peptide to lipid and cellular membranes using methods of fluorescence spectroscopy, lifetime measurement, and microscopy. Our approach offers new possibilities for preparing biologically active liposomes and manipulating living cells.
| Author details: | Ivan Haralampiev, Monique MertensGND, Roland Schwarzer, Andreas HerrmannORCiD, Rudolf Volkmer, Pablo WessigORCiDGND, Peter Mueller |
|---|---|
| DOI: | https://doi.org/10.1002/anie.201408089 |
| ISSN: | 1433-7851 |
| ISSN: | 1521-3773 |
| Pubmed ID: | https://pubmed.ncbi.nlm.nih.gov/25417776 |
| Title of parent work (English): | Angewandte Chemie : a journal of the Gesellschaft Deutscher Chemiker ; International edition |
| Publisher: | Wiley-VCH |
| Place of publishing: | Weinheim |
| Publication type: | Article |
| Language: | English |
| Year of first publication: | 2015 |
| Publication year: | 2015 |
| Release date: | 2017/03/27 |
| Tag: | liposomes; maleimide; membranes; palmitic acid; palmitoylation; peptides |
| Volume: | 54 |
| Issue: | 1 |
| Number of pages: | 4 |
| First page: | 323 |
| Last Page: | 326 |
| Organizational units: | Mathematisch-Naturwissenschaftliche Fakultät / Institut für Chemie |
| Peer review: | Referiert |
