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Semiconductor quantum dots as FRET acceptors for multiplexed diagnostics and molecular ruler application

  • Applications based on Forster resonance energy transfer (FRET) play an important role for the determination of concentrations and distances within nanometer-scale systems in vitro and in vivo in many fields of biotechnology. Semiconductor nanocrystals (Quantum dots - QDs) possess ideal properties for their application as FRET acceptors when the donors have long excited state lifetimes and when direct excitation of QDs can be efficiently suppressed. Therefore, luminescent terbium complexes (LTCs) with excited state lifetimes of more than 2 ms are ideal FRET donor candidates for QD-acceptors. This chapter will give a short overview of theoretical and practical background of FRET, QDs and LTCs, and present some recent applications of LTC-QD FRET pairs for multiplexed ultra-sensitive in vitro diagnostics and nanometer-resolution molecular distance measurements.

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Author:Niko Hildebrandt, Daniel Geissler
Parent Title (English):Advances in Experimental Medicine and Biology
Place of publication:Dordrecht
Editor:E Zahavy, A Ordentlich, S Yitzhaki, A Shafferman
Document Type:Article
Year of first Publication:2012
Year of Completion:2012
Release Date:2017/03/26
Tag:Diagnostics; FRET; Imaging; Quantum dots; Terbium
First Page:75
Last Page:86
Funder:European Commission [NANOGNOSTICS-HEALTH-F5-2009-242264]
Organizational units:Mathematisch-Naturwissenschaftliche Fakultät / Institut für Chemie
Peer Review:Referiert