Identification of two Arabidopsis thaliana plasma membrane transporters able to transport glucose 1-phosphate
- Primary carbohydrate metabolism in plants includes several sugar and sugar-derivative transport processes. Over recent years, evidences have shown that in starch-related transport processes, in addition to glucose 6-phosphate, maltose, glucose and triose-phosphates, glucose 1-phosphate also plays a role and thereby increases the possible fluxes of sugar metabolites in planta. In this study, we report the characterization of two highly similar transporters, At1g34020 and At4g09810, in Arabidopsis thaliana, which allow the import of glucose 1-phosphate through the plasma membrane. Both transporters were expressed in yeast and were biochemically analyzed to reveal an antiport of glucose 1-phosphate/phosphate. Furthermore, we showed that the apoplast of Arabidopsis leaves contained glucose 1-phosphate and that the corresponding mutant of these transporters had higher glucose 1-phosphate amounts in the apoplast and alterations in starch and starch-related metabolism.
Author details: | Irina MalinovaORCiDGND, Stella Kössler, Tom Orawetz, Ulrike Matthes, Slawomir OrzechowskiORCiD, Anke Koch, Jörg FettkeORCiDGND |
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DOI: | https://doi.org/10.1093/pcp/pcz206 |
ISSN: | 0032-0781 |
ISSN: | 1471-9053 |
Pubmed ID: | https://pubmed.ncbi.nlm.nih.gov/31722406 |
Title of parent work (English): | Plant & cell physiology |
Publisher: | Oxford University Press |
Place of publishing: | Oxford |
Publication type: | Article |
Language: | English |
Date of first publication: | 2020/02/02 |
Publication year: | 2019 |
Release date: | 2024/01/10 |
Tag: | Arabidopsis thaliana; apoplast; glucose 1-phosphate transport; starch metabolism; sugar transport |
Volume: | 61 |
Issue: | 2 |
Number of pages: | 12 |
First page: | 381 |
Last Page: | 392 |
Funding institution: | Deutsche ForschungsgemeinschaftGerman Research Foundation (DFG) [DFG-FE; 1030/2-1] |
Organizational units: | Mathematisch-Naturwissenschaftliche Fakultät / Institut für Biochemie und Biologie |
DDC classification: | 5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie |
Peer review: | Referiert |