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Multichannel bipotentiostat integrated with a microfluidic platform for electrochemical real-time monitoring of cell cultures

  • An electrochemical detection system specifically designed for multi-parameter real-time monitoring of stem cell culturing/differentiation in a microfluidic system is presented. It is composed of a very compact 24-channel electronic board, compatible with arrays of microelectrodes and coupled to a microfluidic cell culture system. A versatile data acquisition software enables performing amperometry, cyclic voltammetry and impedance spectroscopy in each of the 12 independent chambers over a 100 kHz bandwidth with current resolution down to 5 pA for 100 ms measuring time. The design of the platform, its realization and experimental characterization are reported, with emphasis on the analysis of impact of input capacitance (i.e., microelectrode size) and microfluidic pump operation on current noise. Programmable sequences of successive injections of analytes (ferricyanide and dopamine) and rinsing buffer solution as well as the impedimetric continuous tracking for seven days of the proliferation of a colony of PC12 cells are successfullyAn electrochemical detection system specifically designed for multi-parameter real-time monitoring of stem cell culturing/differentiation in a microfluidic system is presented. It is composed of a very compact 24-channel electronic board, compatible with arrays of microelectrodes and coupled to a microfluidic cell culture system. A versatile data acquisition software enables performing amperometry, cyclic voltammetry and impedance spectroscopy in each of the 12 independent chambers over a 100 kHz bandwidth with current resolution down to 5 pA for 100 ms measuring time. The design of the platform, its realization and experimental characterization are reported, with emphasis on the analysis of impact of input capacitance (i.e., microelectrode size) and microfluidic pump operation on current noise. Programmable sequences of successive injections of analytes (ferricyanide and dopamine) and rinsing buffer solution as well as the impedimetric continuous tracking for seven days of the proliferation of a colony of PC12 cells are successfully demonstrated.show moreshow less

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Metadaten
Author:Marco Vergani, Marco Carminati, Giorgio Ferrari, Ettore Landini, Claudia Caviglia, Arto Heiskanen, Clement Comminges, Kinga Zor, David Sabourin, Martin Dufva, Maria Dimaki, Roberto Raiteri, Ursula WollenbergerORCiDGND, Jenny Emneus, Marco Sampietro
DOI:https://doi.org/10.1109/TBCAS.2012.2187783
ISSN:1932-4545 (print)
Parent Title (English):IEEE Transactions on biomedical circuits and systems
Publisher:Inst. of Electr. and Electronics Engineers
Place of publication:Piscataway
Document Type:Article
Language:English
Year of first Publication:2012
Year of Completion:2012
Release Date:2017/03/26
Tag:Electrochemical measurements; impedance spectroscopy; microfluidics; multichannel potentiostat; stem cell monitoring
Volume:6
Issue:5
Pagenumber:10
First Page:498
Last Page:507
Funder:EU FP7 EXCELL project [NMP4-SL-2008-214706]
Organizational units:Mathematisch-Naturwissenschaftliche Fakultät / Institut für Biochemie und Biologie
Peer Review:Referiert