Detecting carbohydrate-lectin interactions using a fluorescent probe based on DBD dyes
- Herein we present an efficient synthesis of a biomimetic probe with modular construction that can be specifically bound by the mannose binding FimH protein - a surface adhesion protein of E. coli bacteria. The synthesis combines the new and interesting DBD dye with the carbohydrate ligand mannose via a Click reaction. We demonstrate the binding to E. coli bacteria over a large concentration range and also present some special characteristics of those molecules that are of particular interest for the application as a biosensor. In particular, the mix-and-measure ability and the very good photo-stability should be highlighted here.
Author details: | Denise Bader, Dennis Tobias KlierGND, C. Hettrich, Frank Fabian BierORCiDGND, Pablo WessigORCiDGND |
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DOI: | https://doi.org/10.1039/c5ay02991k |
ISSN: | 1759-9660 |
ISSN: | 1759-9679 |
Title of parent work (English): | Analytical methods : advancing methods and applications |
Publisher: | Royal Society of Chemistry |
Place of publishing: | Cambridge |
Publication type: | Article |
Language: | English |
Year of first publication: | 2016 |
Publication year: | 2016 |
Release date: | 2020/03/22 |
Volume: | 8 |
Number of pages: | 4 |
First page: | 1235 |
Last Page: | 1238 |
Organizational units: | Mathematisch-Naturwissenschaftliche Fakultät / Institut für Chemie |
Mathematisch-Naturwissenschaftliche Fakultät / Institut für Biochemie und Biologie | |
Peer review: | Referiert |