A role of sphingosine in the intracellular survival of Neisseria gonorrhoeae
- Obligate human pathogenic Neisseria gonorrhoeae are the second most frequent bacterial cause of sexually transmitted diseases. These bacteria invade different mucosal tissues and occasionally disseminate into the bloodstream. Invasion into epithelial cells requires the activation of host cell receptors by the formation of ceramide-rich platforms. Here, we investigated the role of sphingosine in the invasion and intracellular survival of gonococci. Sphingosine exhibited an anti-gonococcal activity in vitro. We used specific sphingosine analogs and click chemistry to visualize sphingosine in infected cells. Sphingosine localized to the membrane of intracellular gonococci. Inhibitor studies and the application of a sphingosine derivative indicated that increased sphingosine levels reduced the intracellular survival of gonococci. We demonstrate here, that sphingosine can target intracellular bacteria and may therefore exert a direct bactericidal effect inside cells.
Author details: | Franziska SolgerGND, Tobias C. KunzGND, Julian Fink, Kerstin PaprotkaGND, Pauline Pfister, Franziska HagenGND, Fabian SchumacherORCiDGND, Burkhard KleuserORCiDGND, Jürgen SeibelORCiDGND, Thomas RudelORCiDGND |
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DOI: | https://doi.org/10.3389/fcimb.2020.00215 |
ISSN: | 2235-2988 |
Pubmed ID: | https://pubmed.ncbi.nlm.nih.gov/32477967 |
Title of parent work (English): | Frontiers in Cellular and Infection Microbiology |
Publisher: | Frontiers Media |
Place of publishing: | Lausanne |
Publication type: | Article |
Language: | English |
Date of first publication: | 2020/05/12 |
Publication year: | 2019 |
Release date: | 2023/12/11 |
Tag: | Neisseria gonorrhoeae; click chemistry; invasion; sphingolipids; sphingosine; sphingosine kinases; survival |
Volume: | 10 |
Article number: | 215 |
Number of pages: | 12 |
Funding institution: | Deutsche Forschungsgemeinschaft (DFG)German Research Foundation (DFG); [FOR2123]; DFGGerman Research Foundation (DFG)European Commission; University of Wuerzburg |
Organizational units: | Mathematisch-Naturwissenschaftliche Fakultät / Institut für Ernährungswissenschaft |
DDC classification: | 5 Naturwissenschaften und Mathematik / 54 Chemie / 540 Chemie und zugeordnete Wissenschaften |
Peer review: | Referiert |
Publishing method: | Open Access / Gold Open-Access |
DOAJ gelistet | |
License (German): | CC-BY - Namensnennung 4.0 International |