• search hit 2 of 3
Back to Result List

Proper experimental design requires randomization/balancing of molecular ecology experiments

  • Properly designed (randomized and/or balanced) experiments are standard in ecological research. Molecular methods are increasingly used in ecology, but studies generally do not report the detailed design of sample processing in the laboratory. This may strongly influence the interpretability of results if the laboratory procedures do not account for the confounding effects of unexpected laboratory events. We demonstrate this with a simple experiment where unexpected differences in laboratory processing of samples would have biased results if randomization in DNA extraction and PCR steps do not provide safeguards. We emphasize the need for proper experimental design and reporting of the laboratory phase of molecular ecology research to ensure the reliability and interpretability of results.

Download full text files

  • SHA-1: afa51930ad12a3701eb0a65d30c55b5668d7326e

Export metadata

Additional Services

Share in Twitter Search Google Scholar Statistics
Author:Miklós BálintORCiD, Orsolya Márton, Marlene Schatz, Rolf-Alexander Düring, Hans-Peter GrossartORCiDGND
Parent Title (English):Postprints der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe
Series (Serial Number):Postprints der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe (616)
Document Type:Postprint
Date of first Publication:2019/02/18
Year of Completion:2018
Publishing Institution:Universität Potsdam
Release Date:2019/02/18
Tag:DNA extraction; PCR; batch effect; bias; environmental DNA; laboratory practice; lake community; metabarcoding; nondemonic intrusions; sediment
Source:Ecology and Evolution 8 (2018), pp. 1786–1793 DOI 10.1002/ece3.3687
Organizational units:Mathematisch-Naturwissenschaftliche Fakultät
Dewey Decimal Classification:5 Naturwissenschaften und Mathematik / 50 Naturwissenschaften / 500 Naturwissenschaften und Mathematik
5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie
Peer Review:Referiert
Publication Way:Open Access
Licence (German):License LogoCreative Commons - Namensnennung, 4.0 International