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Molecularly imprinted electropolymer for a hexameric heme protein with direct electron transfer and peroxide electrocatalysis

  • For the first time a molecularly imprinted polymer (MIP) with direct electron transfer (DET) and bioelectrocatalytic activity of the target protein is presented. Thin films of MIPs for the recognition of a hexameric tyrosine-coordinated heme protein (HTHP) have been prepared by electropolymerization of scopoletin after oriented assembly of HTHP on a self-assembled monolayer (SAM) of mercaptoundecanoic acid (MUA) on gold electrodes. Cavities which should resemble the shape and size of HTHP were formed by template removal. Rebinding of the target protein sums up the recognition by non-covalent interactions between the protein and the MIP with the electrostatic attraction of the protein by the SAM. HTHP bound to the MIP exhibits quasi-reversible DET which is reflected by a pair of well pronounced redox peaks in the cyclic voltammograms (CVs) with a formal potential of −184.4 ± 13.7 mV vs. Ag/AgCl (1 M KCl) at pH 8.0 and it was able to catalyze the cathodic reduction of peroxide. At saturation the MIP films show a 12-fold higherFor the first time a molecularly imprinted polymer (MIP) with direct electron transfer (DET) and bioelectrocatalytic activity of the target protein is presented. Thin films of MIPs for the recognition of a hexameric tyrosine-coordinated heme protein (HTHP) have been prepared by electropolymerization of scopoletin after oriented assembly of HTHP on a self-assembled monolayer (SAM) of mercaptoundecanoic acid (MUA) on gold electrodes. Cavities which should resemble the shape and size of HTHP were formed by template removal. Rebinding of the target protein sums up the recognition by non-covalent interactions between the protein and the MIP with the electrostatic attraction of the protein by the SAM. HTHP bound to the MIP exhibits quasi-reversible DET which is reflected by a pair of well pronounced redox peaks in the cyclic voltammograms (CVs) with a formal potential of −184.4 ± 13.7 mV vs. Ag/AgCl (1 M KCl) at pH 8.0 and it was able to catalyze the cathodic reduction of peroxide. At saturation the MIP films show a 12-fold higher electroactive surface concentration of HTHP than the non-imprinted polymer (NIP).show moreshow less

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Metadaten
Author:Lei Peng, Aysu Yarman, Katharina J. Jetzschmann, Jae-Hun Jeoung, Daniel SchadORCiDGND, Holger Dobbek, Ursula WollenbergerORCiDGND, Frieder W. SchellerORCiDGND
URN:urn:nbn:de:kobv:517-opus4-400627
Series (Serial Number):Postprints der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe (362)
Document Type:Postprint
Language:English
Date of first Publication:2017/09/22
Year of Completion:2017
Publishing Institution:Universität Potsdam
Release Date:2017/09/22
Tag:bioelectrocatalysis; direct electron transfer; hydrogen peroxide; molecularly imprinted polymers; self-assembled monolayer
Pagenumber:11
Source:Sensors 16 (2016) Nr. 3. - DOI: 10.3390/s16030272
Organizational units:Mathematisch-Naturwissenschaftliche Fakultät / Institut für Biochemie und Biologie
Dewey Decimal Classification:6 Technik, Medizin, angewandte Wissenschaften / 62 Ingenieurwissenschaften / 620 Ingenieurwissenschaften und zugeordnete Tätigkeiten
Peer Review:Referiert
Publication Way:Open Access
Grantor:Multidisciplinary Digital Publishing Institute (MDPI)
Licence (German):License LogoCreative Commons - Namensnennung, 4.0 International