Monitoring hormone-induced oxygen consumption in the salivary glands of the blowfly, Calliphora vicina, by use of luminescent microbeads
- The salivary glands of the blowfly were injected with luminescent oxygen-sensitive microbeads. The changes in oxygen content within individual gland tubules during hormone-induced secretory activity were quantified. The measurements are based on an upgraded phase-modulation technique, where the phase shift of the sensor phosphorescence is determined independently from concentration and background signals. We show that the combination of a lock-in amplifier with a fluorescence microscope results in a convenient setup to measure oxygen concentrations within living animal tissues at the cellular level.
| Verfasserangaben: | Elmar Schmälzlin, Bernd Walz, Ingo Klimant, Bettina Schewe, Hans-Gerd LöhmannsröbenORCiDGND |
|---|---|
| URN: | urn:nbn:de:kobv:517-opus-12206 |
| Schriftenreihe (Bandnummer): | Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe (paper 006) |
| Publikationstyp: | Postprint |
| Sprache: | Englisch |
| Erscheinungsjahr: | 2006 |
| Veröffentlichende Institution: | Universität Potsdam |
| Datum der Freischaltung: | 26.01.2007 |
| Quelle: | Sensors and Actuators B: Chemical. - ISSN 0925-4005. - 119 (2006), 1 , p. 251 - 254 |
| Organisationseinheiten: | Mathematisch-Naturwissenschaftliche Fakultät / Institut für Chemie |
| DDC-Klassifikation: | 5 Naturwissenschaften und Mathematik / 54 Chemie / 540 Chemie und zugeordnete Wissenschaften |
| Externe Anmerkung: | first published in: Sensors and Actuators B: Chemical. - ISSN 0925-4005. - 119 (2006), 1, p. 251 - 254 doi: 10.1016/j.snb.2005.12.024 |
