The search result changed since you submitted your search request. Documents might be displayed in a different sort order.
  • search hit 1 of 12
Back to Result List

Proper experimental design requires randomization/balancing of molecular ecology experiments

  • Properly designed (randomized and/or balanced) experiments are standard in ecological research. Molecular methods are increasingly used in ecology, but studies generally do not report the detailed design of sample processing in the laboratory. This may strongly influence the interpretability of results if the laboratory procedures do not account for the confounding effects of unexpected laboratory events. We demonstrate this with a simple experiment where unexpected differences in laboratory processing of samples would have biased results if randomization in DNA extraction and PCR steps do not provide safeguards. We emphasize the need for proper experimental design and reporting of the laboratory phase of molecular ecology research to ensure the reliability and interpretability of results.

Download full text files

  • SHA-1: afa51930ad12a3701eb0a65d30c55b5668d7326e

Export metadata

Additional Services

Search Google Scholar Statistics
Metadaten
Author details:Miklós BálintORCiD, Orsolya Márton, Marlene Schatz, Rolf-Alexander Düring, Hans-Peter GrossartORCiDGND
URN:urn:nbn:de:kobv:517-opus4-423878
DOI:https://doi.org/10.25932/publishup-42387
ISSN:1866-8372
Title of parent work (English):Postprints der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe
Publication series (Volume number):Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe (616)
Publication type:Postprint
Language:English
Date of first publication:2019/02/18
Publication year:2018
Publishing institution:Universität Potsdam
Release date:2019/02/18
Tag:DNA extraction; PCR; batch effect; bias; environmental DNA; laboratory practice; lake community; metabarcoding; nondemonic intrusions; sediment
Issue:616
Number of pages:8
Source:Ecology and Evolution 8 (2018), pp. 1786–1793 DOI 10.1002/ece3.3687
Organizational units:Mathematisch-Naturwissenschaftliche Fakultät
DDC classification:5 Naturwissenschaften und Mathematik / 50 Naturwissenschaften / 500 Naturwissenschaften und Mathematik
5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie
Peer review:Referiert
Publishing method:Open Access
License (German):License LogoCC-BY - Namensnennung 4.0 International
External remark:Bibliographieeintrag der Originalveröffentlichung/Quelle
Accept ✔
This website uses technically necessary session cookies. By continuing to use the website, you agree to this. You can find our privacy policy here.