A pH Responsive Redox Hydrogel for Electrochemical Detection of Redox Silent Biocatalytic Processes. Control of Hydrogel Solvation
- The control of bioelectrocatalytic processes by external stimuli for the indirect detection of non-redox active species was achieved using an esterase and a redox enzyme both integrated within a redox hydrogel. The poly( vinyl) imidazole Os(bpy)(2)Cl hydrogel displays pH-responsive properties. The esterase catalysed reaction leads to a local pH decrease causing protonation of imidazole moieties thus increasing hydrogel solvation and mobility of the tethered Os-complexes. This is the key step to enable improved electron transfer between an aldehyde oxidoreductase and the polymer-bound Os-complexes. The off-on switch is further integrated in a biofuel cell system for self-powered signal generation.
| Author details: | Andrea Contin, Stefano Frasca, Jeevanthi Vivekananthan, Silke LeimkühlerORCiDGND, Ursula WollenbergerORCiDGND, Nicolas Plumere, Wolfgang Schuhmann |
|---|---|
| DOI: | https://doi.org/10.1002/elan.201400621 |
| ISSN: | 1040-0397 |
| ISSN: | 1521-4109 |
| Title of parent work (English): | Electroanalysis : an international journal devoted to fundamental and practical aspects of electroanalysis |
| Publisher: | Wiley-VCH |
| Place of publishing: | Weinheim |
| Publication type: | Article |
| Language: | English |
| Year of first publication: | 2015 |
| Publication year: | 2015 |
| Release date: | 2017/03/27 |
| Tag: | Biofuel cell; External stimuli; Self-powered biosensor; Solvation; pH responsive hydrogel |
| Volume: | 27 |
| Issue: | 4 |
| Number of pages: | 7 |
| First page: | 938 |
| Last Page: | 944 |
| Funding institution: | Cluster of Excellence RESOLV - Deutsche Forschungsgemeinschaft (DFG) [EXC 1069]; Unicat - Deutsche Forschungsgemeinschaft (DFG) [EXC 314/2]; Bundesministerium fur Bildung und Forschung (BMBF) within the Project Taschentuchlabor [03IS2201B, 03IS2201F] |
| Organizational units: | Mathematisch-Naturwissenschaftliche Fakultät / Institut für Biochemie und Biologie |
| Peer review: | Referiert |
