Second harmonic generation microscopy investigation of the crystalline ultrastructure of three barley starch lines affected by hydration
- Second harmonic generation (SHG) microscopy is employed to study changes in crystalline organization due to altered gene expression and hydration in barley starch granules. SHG intensity and susceptibility ratio values (R'(SHG)) are obtained using reduced Stokes-Mueller polarimetric microscopy. The maximum R'(SHG) values occur at moderate moisture indicating the narrowest orientation distribution of nonlinear dipoles from the cylindrical axis of glucan helices. The maximum SHG intensity occurs at the highest moisture and amylopectin content. These results support the hypothesis that SHG is caused by ordered hydrogen and hydroxyl bond networks which increase with hydration of starch granules. (C) 2015 Optical Society of America
| Author details: | Richard Cisek, Danielle Tokarz, Martin SteupORCiDGND, Ian J. Tetlow, Michael J. Emes, Kim H. Hebelstrup, Andreas Blennow, Virginijus Barzda |
|---|---|
| DOI: | https://doi.org/10.1364/BOE.6.003694 |
| ISSN: | 2156-7085 |
| Pubmed ID: | https://pubmed.ncbi.nlm.nih.gov/26504621 |
| Title of parent work (English): | Biomedical optics express |
| Publisher: | Optical Society of America |
| Place of publishing: | Washington |
| Publication type: | Article |
| Language: | English |
| Year of first publication: | 2015 |
| Publication year: | 2015 |
| Release date: | 2017/03/27 |
| Volume: | 6 |
| Issue: | 10 |
| Number of pages: | 7 |
| First page: | 3694 |
| Last Page: | 3700 |
| Funding institution: | Natural Sciences and Engineering Research Council of Canada (NSERC) |
| Organizational units: | Mathematisch-Naturwissenschaftliche Fakultät / Institut für Biochemie und Biologie |
| Peer review: | Referiert |
| Publishing method: | Open Access |
